Biochip/laser cell deposition system to assess polarized axonal growth from single neurons and neuron/glia pairs in microchannels with novel asymmetrical geometries
Biochip/laser cell deposition system to assess polarized axonal growth from single neurons and neuron/glia pairs in microchannels with novel asymmetrical geometries
复制标题
生物芯片/激光细胞沉积系统，用于评估具有新颖不对称几何形状的微通道中单个神经元和神经元/胶质细胞对的偏振轴突生长
DOI：
10.1063/1.3552998
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发表时间：
2011-03-01
期刊：
影响因子：
3.2
通讯作者：
Gao, B. Z.
中科院分区：
文献类型：
--
作者：
Pirlo, R. K.;Sweeney, A. J.;Gao, B. Z.
轴突寻路在正常和致病性大脑发育以及神经再生医学中发挥着重要作用。在这两种情况下，轴突生长都受到微环境的影响，包括可溶性分子以及来自引导细胞和细胞基质的接触介导的信号传导。微流体装置是在单细胞水平上创建微环境的强大工具。在本文中，开发了一种基于不对称通道的生物芯片，该芯片随后可以合并到用于神经元网络研究的微流体装置中，以研究几何形状以及支持极化轴突生长的细胞控制以形成确定的神经元电路。使用激光细胞沉积系统将单个细胞（包括神经元-胶质细胞对）放入该装置的特定微孔中，从而使轴突生长不受亲细胞/疏性表面模式的影响。相差显微镜显示，一种新颖的“阻碍”通道结构对轴突在预期方向上的生长的影响比相反方向的影响为 4:1。在异型实验中，通过延时显微镜观察神经胶质细胞对轴突生长路径的影响。因此，表明可以在激光图案生物芯片中开发单细胞和异型神经元寻路模型。 (C) 2011 年美国物理研究所。 [号码：10.1063/1.3552998]
Axon path-finding plays an important role in normal and pathogenic brain development as well as in neurological regenerative medicine. In both scenarios, axonal growth is influenced by the microenvironment including the soluble molecules and contact-mediated signaling from guiding cells and cellular matrix. Microfluidic devices are a powerful tool for creating a microenvironment at the single cell level. In this paper, an asymmetrical-channel-based biochip, which can be later incorporated into microfluidic devices for neuronal network study, was developed to investigate geometric as well as supporting cell control of polarized axonal growth in forming a defined neuronal circuitry. A laser cell deposition system was used to place single cells, including neuron-glia pairs, into specific microwells of the device, enabling axonal growth without the influence of cytophilic/phobic surface patterns. Phase microscopy showed that a novel "snag" channel structure influenced axonal growth in the intended direction 4:1 over the opposite direction. In heterotypic experiments, glial cell influence over the axonal growth path was observed with time-lapse microscopy. Thus, it is shown that single cell and heterotypic neuronal path-finding models can be developed in laser patterned biochips. (C) 2011 American Institute of Physics. [doi: 10.1063/1.3552998]