GAMMA-TUBULIN IS A HIGHLY CONSERVED COMPONENT OF THE CENTROSOME
GAMMA-TUBULIN IS A HIGHLY CONSERVED COMPONENT OF THE CENTROSOME
复制标题
DOI：
10.1016/0092-8674(91)90390-k
复制
发表时间：
1991-05-31
期刊：
影响因子：
64.5
通讯作者：
KIRSCHNER, M
中科院分区：
文献类型：
--
作者：
STEARNS, T;EVANS, L;KIRSCHNER, M
我们克隆并鉴定了来自 X. laevis 和 S. pombe 的 γ-微管蛋白基因，以及来自玉米、硅藻和芽殖酵母的部分基因。 这些基因编码的蛋白质彼此非常相似，并且与原始曲霉属蛋白质非常相似，表明γ-微管蛋白是微管蛋白家族中普遍存在且高度保守的亚家族。 粟酒裂殖酵母基因的无效突变是致命的。 γ-微管蛋白是一种次要蛋白质，其含量低于 α-和 β-微管蛋白的 1%，并且仅限于中心体。 特别地，γ-微管蛋白与中心粒周围材料、中心体的微管成核材料相关。 当微管解聚时，γ-微管蛋白仍然与中心体相关，这表明它是可能在微管组织中发挥作用的组成部分。
We have cloned and characterized gamma-tubulin genes from both X. laevis and S. pombe, and partial genes from maize, diatom, and a budding yeast. The proteins encoded by these genes are very similar to each other and to the original Aspergillus protein, indicating that gamma-tubulins are an ubiquitous and highly conserved subfamily of the tubulin family. A null mutation of the S. pombe gene is lethal. Gamma-tubulin is a minor protein, present at less than 1% the level of alpha- and beta-tubulin, and is limited to the centrosome. In particular, gamma-tubulin is associated with the pericentriolar material, the microtubule-nucleating material of the centrosome. Gamma-tubulin remains associated with the centrosome when microtubules are depolymerized, suggesting that it is an integral component that might play a role in microtubule organization.