An iron-sulfur domain of the eukaryotic primase is essential for RNA primer synthesis.
An iron-sulfur domain of the eukaryotic primase is essential for RNA primer synthesis.
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DOI：
10.1038/nsmb1288
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发表时间：
2007-09
影响因子：
16.8
通讯作者：
Pellegrini, Luca
中科院分区：
文献类型：
--
作者：
Klinge, Sebastian;Hirst, Judy;Maman, Joseph D;Krude, Torsten;Pellegrini, Luca
引物酶合成亲本 DNA 链复制所需的 RNA 引物。在这里，我们报道异二聚体古菌/真核引物酶是一种铁硫（Fe-S）蛋白。 Fe-S 簇的结合是由大亚基 C 末端的进化保守结构域介导的。我们进一步表明，Fe-S 结构域对于真核引物酶启动 DNA 复制的独特能力至关重要。
Primases synthesise the RNA primers that are necessary for replication of the parental DNA strands. Here we report that the heterodimeric archaeal/eukaryotic primase is an iron-sulfur (Fe-S) protein. Binding of the Fe-S cluster is mediated by an evolutionarily conserved domain at the C terminus of the large subunit. We further show that the Fe-S domain is essential to the unique ability of the eukaryotic primase to start DNA replication.