PHOSPHOLIPID SUBSTITUTION OF CAPSULAR POLYSACCHARIDES AND MECHANISMS OF CAPSULE FORMATION IN NEISSERIA-MENINGITIDIS
PHOSPHOLIPID SUBSTITUTION OF CAPSULAR POLYSACCHARIDES AND MECHANISMS OF CAPSULE FORMATION IN NEISSERIA-MENINGITIDIS
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DOI：
10.1111/j.1365-2958.1993.tb01592.x
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发表时间：
1993-05-01
影响因子：
3.6
通讯作者：
MULLER, A
中科院分区：
文献类型：
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作者：
FROSCH, M;MULLER, A
在脑膜炎奈瑟菌的荚膜基因复合体 (cps) 内，两个功能区 B 和 C 参与细胞质合成的荚膜多糖的表面易位，荚膜多糖是 α-2,8 聚神经氨酸的均聚物。 C 区基因产物与主动转运蛋白 ABC（ATP 结合盒）超家族的转运蛋白具有相同的特征。为了分析 B 区在荚膜多糖表面易位中的作用，我们通过亲和层析纯化了 B 区和 C 区缺陷型大肠杆菌克隆的多糖。通过凝胶过滤测定多糖的分子量，并通过聚丙烯酰胺凝胶电泳和免疫印迹分析多糖的磷脂取代。结果表明，具有磷脂锚的全尺寸荚膜多糖是在细胞内合成的，并且脂质修饰是多糖易位至细胞表面的强烈要求。 B 区编码的蛋白质参与荚膜多糖的磷脂取代。 B区的核苷酸序列分析揭示了两个开放阅读框，编码分子量为45.1和48.7 kDa的蛋白质。
Within the capsule gene complex (cps) of Neisseria meningitidis two functional regions B and C are involved in surface translocation of the cytoplasmically synthesized capsular polysaccharide, which is a homopolymer of alpha-2,8 polyneuraminic acid. The region-C gene products share characteristics with transporter proteins of the ABC (ATP-binding cassette) superfamily of active transporters. For analysis of the role of region B in surface translocation of the capsular polysaccharide we purified the polycaccharides of region B- and region C-defective Escherichia coli clones by affinity chromatography. The molecular weights of the polysaccharides were determined by gel filtration and the polysaccharides were analysed for phospholipid substitution by polyacrylamide gel electrophoresis and immunoblotting. The results indicate that the full-size capsular polysaccharide with a phospholipid anchor is synthesized intracellularly and that lipid modification is a strong requirement for translocation of the polysaccharide to the cell surface. Proteins encoded by region B are involved in phospholipid substitution of the capsular polysaccharide. Nucleotide sequence analysis of region B revealed two open reading frames, which encode proteins with molecular masses of 45.1 and 48.7 kDa.