RELAPSE VERSUS REINFECTION WITH CLOSTRIDIUM-DIFFICILE
RELAPSE VERSUS REINFECTION WITH CLOSTRIDIUM-DIFFICILE
复制标题
DOI：
10.1017/s0950268800049323
复制
发表时间：
1991-12-01
影响因子：
4.2
通讯作者：
RILEY, TV
中科院分区：
文献类型：
--
作者：
ONEILL, GL;BEAMAN, MH;RILEY, TV
艰难梭菌相关性腹泻的复发发生在15%-20%的患者中；然而，复发是由于机体的内源性来源还是由于环境的再次感染尚不清楚。用限制性内切酶分析(REA)对10例明显复发的患者的多重分离株进行分型。超过一半的复发是由于感染了一种新的艰难梭菌。其余患者感染了相同的菌株，但无法确定该菌株是从环境中获得的还是从内源性来源获得的。如果一个人的胃肠道中含有一种以上的艰难梭菌，则可能会发生不同菌株的复发。为了调查这种可能性，对另外10名患者进行了多种菌株携带者的评估。来自每个患者的原代培养板上的10个菌落被REA分型，并测试它们产生细胞毒素的能力。同一患者的所有分离株在两种方法下都是相同的，这表明多重携带菌株可能是罕见的事件。
Relapse of Clostridium difficile-associated diarrhoea occurs in 15-20% of patients; however, whether relapse is due to an endogenous source of the organism or reinfection from the environment remains unclear. Restriction enzyme analysis (REA) of chromosomal DNA was used to type multiple isolates from ten patients who had experienced apparent relapses. More than half the relapses were due to infection with a new strain of C. difficile. The remaining patients were infected with the same strain, but whether this strain was acquired from the environment or from endogenous sources could not be determined. Relapses with a different strain of C. difficle could occur if an individual harboured more than one strain in their gastrointestinal tract. To investigate this possibility ten other patients were assessed for carriage of multiple strains. Ten colonies from a primary culture plate from each patient were typed by REA and tested for their ability to produce cytotoxin. All isolates from the same patient were identical by both methods, indicating that multiple carriage of strains may be a rare event.