Evidence for ovarian "inhibin": suppression of the secondary rise in serum follicle stimulating hormone levels in proestrous rats by injection of porcine follicular fluid.
Evidence for ovarian "inhibin": suppression of the secondary rise in serum follicle stimulating hormone levels in proestrous rats by injection of porcine follicular fluid.
复制标题
卵巢“抑制素”的证据：通过注射猪卵泡液抑制发情前大鼠血清卵泡刺激素水平的继发性升高。
DOI：
--
复制
发表时间：
1977
影响因子：
11.1
通讯作者：
C. Channing
中科院分区：
文献类型：
--
作者：
N. Schwartz;C. Channing
本研究旨在观察猪卵泡液注入大鼠体内后是否能抑制血清促卵泡激素（FSH）水平的升高。对于这些研究，选择戊巴比妥处理的发情前期大鼠作为主要试验动物模型。如果对这些大鼠给予促黄体生成素（LH）的人工激增，它可以诱导FSH分泌率的同步二次升高。正常盐水处理的大鼠也用作试验动物。它们表现出排卵前LH和FSH的内源性“激增”，以及继发性FSH升高。从中型和大型（直径3- 10 mm）卵泡中采集猪卵泡液，并用活性炭处理以去除内源性类固醇。活性炭处理的猪血清作为对照溶液。在LH注射后立即和3小时将液体以两个0.5 ml剂量腹腔注射到戊巴比妥治疗的发情前期大鼠中。卵泡液而非血清以剂量依赖性方式抑制LH诱导的继发性FSH升高（P < 0.01），但不改变LH对血清卵巢类固醇水平或卵泡破裂的影响。总剂量低至200穆尔时有效。猪卵泡液也阻断了正常发情前期大鼠的继发性FSH峰，表现出内源性LH/FSH初级峰。因此，看来猪卵泡液含有一种非甾体物质，可阻断继发于天然或人工LH峰的FSH分泌。
The present studies were carried out to see if porcine follicular fluid could inhibit increases in serum follicle stimulating hormone (FSH) levels when injected into the rat. For these studies the pentobarbital-treated proestrous rat was chosen as the major test animal model. If an artificial surge of luteinizing hormone (LH) is administered to these rats, it can induce a synchronized secondary rise in FSH secretion rate. Normal saline-treated rats were also used as test animals. They exhibit preovulatory endogenous "surges" of LH and FSH, and also a secondary FSH rise. Porcine follicular fluid was harvested from medium-sized and large (3- to 10-mm diameter) follicles and treated with charcoal to remove endogenous steroids. Charcoal-treated porcine serum served as a control solution. The fluid was injected intraperitoneally in two 0.5-ml doses into pentobarbital-treated proestrous rats immediately and 3 hr after LH injection. Follicular fluid, but not the serum, suppressed the secondary, LH-induced FSH rise (P < 0.01) in a dose-dependent manner, without altering the effects of LH upon serum ovarian steroid levels or follicular rupture. It was effective down to a total dose of 200 mul. Porcine follicular fluid also blocked the secondary FSH surge in normal proestrous rats exhibiting endogenous LH/FSH primary surges. Thus, it would appear that porcine follicular fluid contains a non-steroidal substance(s) that can block the secretion of FSH that is secondary to a natural or artificial LH surge.