Silkworm and spider silk scaffolds for chondrocyte support
Silkworm and spider silk scaffolds for chondrocyte support
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DOI：
10.1007/s10856-008-3474-6
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发表时间：
2008-11-01
影响因子：
3.7
通讯作者：
Verbruggen, Gust
中科院分区：
文献类型：
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作者：
Gellynck, Kris;Verdonk, Peter C. M.;Verbruggen, Gust
目的用家蚕茧、蜘蛛卵囊和蜘蛛牵引丝纤维构建支架，并检测其对软骨细胞的附着和支撑作用。方法用家蚕茧、大腹蜘蛛卵囊和牵引丝纤维制成3种不同的支架材料。研究了人关节软骨细胞在这些生物蛋白基质上的附着情况。软骨细胞产生细胞外基质，免疫组织化学染色观察。此外，还研究了压缩行为与孔隙率的关系。结果蚕丝支架的压缩模量值与其孔隙率有关。软骨细胞能够在不同的纤维和支架上附着和生长数周，同时产生细胞外基质产品。结论以家蚕丝和蜘蛛丝为原料可制成多孔支架，用于软骨再生。力学性能与结构的孔隙率和孔大小有关。细胞的铺展和细胞的表达取决于孔隙率和孔径。
Objective To create scaffolds with silkworm cocoon, spider egg sac and spider dragline silk fibres and examine their use for chondrocyte attachment and support. Methods Three different kinds of scaffolds were developed with Bombyx mori cocoon, Araneus diadematus egg sac and dragline silk fibres. The attachment of human articular cartilage cells were investigated on these bioprotein matrices. The chondrocytes produced an extracellular matrix which was studied by immunostaining. Moreover, the compression behaviour in relation to the porosity was studied. Results The compression modulus of a silkworm silk scaffold was related to its porosity. Chondrocytes were able to attach and to grow on the different fibres and in the scaffolds for several weeks while producing extracellular matrix products. Conclusion Porous scaffolds can be made out of silkworm and spider silk for cartilage regeneration. Mechanical properties are related to porosity and pore size of the construct. Cell spreading and cell expression depended on the porosity and pore-size.