THE INDUCTION OF ADIPOSE CONVERSION BY BEZAFIBRATE IN 3T3-L1 CELLS - SYNERGISM WITH DIBUTYRYL-CAMP
THE INDUCTION OF ADIPOSE CONVERSION BY BEZAFIBRATE IN 3T3-L1 CELLS - SYNERGISM WITH DIBUTYRYL-CAMP
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DOI：
10.1016/0167-4889(90)90244-8
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发表时间：
1990-09-01
期刊：
影响因子：
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通讯作者：
BARTANA, J
中科院分区：
文献类型：
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作者：
BRANDES, R;ARAD, R;BARTANA, J
苯扎贝特(Brandes，R.，Hertz，R.，Arad，R.，Naishtat，S.，Weil，S.和Bar-Tana，J.(1987)Life Sci.，40,935-941)对3T3-L1细胞脂肪转化的诱导作用可被双丁酰cAMP以及福司可林、茶碱或异丁基甲基黄嘌呤与苯扎贝特诱导剂一起增强。苯扎贝特和二丁酰cAMP的协同作用导致脂肪转化的晚期标志物，如脂肪堆积或甘油-3-磷酸脱氢酶活性及其mRNA的表达增强。这种晚期标记的增强表达反映在缩短了它们首次出现所需的时间，以及在脂肪转化过程中增加了它们的产量。随着谷氨酰胺合成酶mRNA的积累作为脂肪转化的早期标志，苯扎贝特和二丁酰cAMP早在加入融合的3T3-L1细胞5h后就已经显示出协同作用。因此，苯扎贝特诱导3T3-L1细胞的脂肪转化似乎涉及到一个早期事件，该事件受到细胞内cAMP可获得性的限制。
The induction of adipose conversion in 3T3-L1 cells by bezafibrate (Brandes, R., Hertz, R., Arad, R., Naishtat, S., Weil, S. and Bar-Tana, J. (1987) Life Sci., 40, 935-941) was enhanced by dibutyryl-cAMP as well as forskolin, theophylline or isobutylmethylxanthine added to the incubation medium together with the bezafibrate inducer. The synergistic effect of bezafibrate and dibutyryl-cAMP resulted in enhancing the expression of late markers of adipose conversion, e.g., lipid accumulation or glycerol-3-phosphate dehydrogenase activity and its mRNA. This enhanced expression of late markers was reflected in shortening the time period required for their first appearance as well as increasing their yield during the course of adipose conversion. By following the accumulation of glutamine synthetase mRNA serving as an early marker for adipose conversion, the synergistic effect of bezafibrate and dibutyryl-cAMP was already evident as early as 5 h following their addition to confluent 3T3-L1 cells. Hence, the induction of adipose conversion by bezafibrate in 3T3-L1 cells appears to involve an early event which is rate-limited by the availability of intracellular cAMP.