Apoptosis of phagocytic cells induced by Candida albicans and production of IL-10
Apoptosis of phagocytic cells induced by Candida albicans and production of IL-10
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DOI：
10.1016/j.femsim.2004.05.006
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发表时间：
2004-10-01
影响因子：
--
通讯作者：
Felipe, I
中科院分区：
文献类型：
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作者：
Gasparoto, TH;Gaziri, LCJ;Felipe, I
白念珠菌CRI株与巨噬细胞体外共孵育后，巨噬细胞早期出现凋亡，2 h后逐渐坏死。本研究探讨了CRI株接种小鼠腹腔后是否引起巨噬细胞凋亡或坏死，以及这是否与IL-10的分泌有关。小鼠腹腔巨噬细胞接种C.白念珠菌CR 1在30 min ~ 2 h内出现凋亡和坏死的现象，而热灭活的白念珠菌则无此现象。IL-10的生产是低的，在第一个6小时感染后，当巨噬细胞占主导地位的腹腔渗出液，而其较高的生产后24小时与渗出液中的中性粒细胞的增加。用胃蛋白酶抑制剂（蛋白酶抑制剂）处理CRI阻止了细胞凋亡的过程并显著降低了IL-10的产生，这表明IL-10的产生增加是由感染的初始阶段期间发生的过程引起的，例如细胞凋亡、坏死和死亡细胞的摄取。(C)2004年，欧洲微生物学会联合会。Elsevier B. V.出版，保留所有权利。
Macrophages co-incubated with Candida albicans strain CRI in vitro showed early signs of apoptosis, but evolved to necrosis after 2 h. In this study, we investigated whether strain CRI caused apoptosis or necrosis of macrophages after its inoculation into mice peritoneal cavity, and whether this correlated with the secretion of IL-10. Peritoneal macrophages from mice that received an inoculum of C. albicans CR 1 showed signs of apoptosis and necrosis from 30 min to 2 h afterwards, whereas heat-killed C albicans did not cause those effects. IL-10 production was low during the first 6 h post-infection, when macrophages predominated in the peritoneal exudate, whereas its higher production after 24 h correlated with an increase of neutrophils in the exudate. Treatment of CRI with pepstatin (an inhibitor of proteinases) prevented the process of apoptosis and significantly reduced IL-10 production, suggesting that the increased production of IL-10 was caused by processes occurring during the initial phase of infection, such as apoptosis, necrosis and uptake of death cells. (C) 2004 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.