Partial purification and functional reconstitution of GDP sensitive brown adipose tissue mitochondria uncoupling protein using octyl glucoside.
Partial purification and functional reconstitution of GDP sensitive brown adipose tissue mitochondria uncoupling protein using octyl glucoside.
复制标题
使用辛基葡萄糖苷对 GDP 敏感的棕色脂肪组织线粒体解偶联蛋白进行部分纯化和功能重建。
DOI：
10.1016/s0006-291x(85)80189-3
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发表时间：
1985
影响因子：
3.1
通讯作者：
Shrago,E
中科院分区：
文献类型：
--
作者：
Strieleman,PJ;Schalinske,KL;Shrago,E
通过用40 mM辛基葡萄糖苷提取棕色脂肪组织线粒体（BATM），然后在ATP-琼脂糖上进行亲和层析，实现了棕色脂肪组织线粒体（BATM）解偶联蛋白的部分纯化。使用辛基葡糖苷透析将分离的蛋白质功能性地重构为脂质体。含有解偶联蛋白的蛋白脂质体在缬氨霉素诱导的钾扩散电位下具有增加的质子或氯离子电导。200 μM GDP可抑制离子电导的增加。
A partial purification of the uncoupling protein of brown adipose tissue mitochondria (BATM) was achieved by extraction of BATM with 40 mM octyl glucoside, followed by affinity chromatography on ATP-agarose. The isolated protein was functionally reconstituted into liposomes using octyl glucoside dialysis. Proteoliposomes containing the uncoupling protein had an increased proton or chloride conductance when subjected to a valinomycin-induced potassium diffusion potential. The increased ion conductance was consistently found to be inhibited by 200 μM GDP.