Histological changes in intestine of Atlantic salmon (Salmo salar L.) following in vitro exposure to pathogenic and probiotic bacterial strains
Histological changes in intestine of Atlantic salmon (Salmo salar L.) following in vitro exposure to pathogenic and probiotic bacterial strains
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DOI：
10.1007/s00441-006-0323-0
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发表时间：
2007-03
影响因子：
3.6
通讯作者：
E. Ringø;E. Ringø;Irene Salinas;Irene Salinas;R. Olsen;A. Nyhaug;Reidar Myklebust;Terry M Mayhew-Terr
中科院分区：
文献类型：
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作者：
E. Ringø;E. Ringø;Irene Salinas;Irene Salinas;R. Olsen;A. Nyhaug;Reidar Myklebust;Terry M Mayhew-Terr
疖病和弧菌病是给养鱼业造成严重经济损失的疾病。将大西洋鲑鱼 (Salmo salarL.) 的前肠在体外暴露于两种鱼类病原体，杀鲑气单胞菌（疖病的病原体）和鳗弧菌（弧菌病的病原体），以及一种益生菌菌株，每毫升 6 × 104 或 6 × 106 个活菌。通过光学和电子显微镜评估细菌暴露后的组织学变化。对照样品（前肠仅暴露于林格氏溶液）和仅暴露于 C 的样品。 divergens 的外观与完整的肠粘膜上皮相似，没有损坏的迹象。然而，前肠暴露于病原菌会导致上皮细胞受损、管腔内细胞碎片和微绒毛紊乱。前肠与病原体共孵育 C. divergens 并没有逆转病原体造成的破坏性影响，尽管使用益生菌时这些破坏性影响得到了缓解。基于这些结果，我们建议益生菌 C. divergens 能够在一定程度上防止病原体引起的大西洋鲑鱼前肠损伤。
Furunculosis and vibriosis are diseases that cause severe economic losses in the fish-farming industry. The foregut of the Atlantic salmon (Salmo salarL.) was exposed in vitro to two fish pathogens,Aeromonas salmonicida(causative agent of furunculosis) andVibrio anguillarum(causative agent of vibriosis), and to one probiotic strain,Carnobacterium divergens, at 6 × 104or 6 × 106viable bacteria per milliliter. Histological changes following bacterial exposure were assessed by light and electron microscopy. Control samples (foregut exposed to Ringer’s solution only) and samples exposed only toC. divergenshad a similar appearance to intact intestinal mucosal epithelium, with no signs of damage. However, exposure of the foregut to the pathogenic bacteria resulted in damaged epithelial cells, cell debris in the lumen, and disorganization of the microvilli. Co-incubation of the foregut with a pathogen andC. divergensdid not reverse the damaging effects caused by the pathogen, although these were alleviated when probiotic bacteria were used. Based on these results, we suggest that the probiotic bacterium,C. divergens, is able to prevent, to some extent, pathogen-induced damage in the Atlantic salmon foregut.