Arachidonic acid metabolism in guinea pig megakaryocytes.
Arachidonic acid metabolism in guinea pig megakaryocytes.
复制标题
豚鼠巨核细胞中的花生四烯酸代谢。
DOI：
10.1016/0006-291x(82)91555-8
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发表时间：
1982
影响因子：
3.1
通讯作者：
Walenga,RW
中科院分区：
文献类型：
--
作者：
Miller,JL;Stuart,MJ;Walenga,RW
将豚鼠巨核细胞分离至75-90%纯度，与1- 14 C-花生四烯酸孵育，并通过薄层色谱法分析放射性代谢物。血栓烷B2、12-羟基十七碳三烯酸（HHT）和12-羟基二十碳四烯酸（12-HETE）是鉴定的主要产物。吲哚美辛预孵育抑制血栓素B2和HHT的产生。对同一动物的巨核细胞和血小板产生的代谢物进行比较，发现一个巨核细胞产生的血栓素B2、HHT或12-HETE是单个血小板的2500-4000倍。这些结果表明，分离的巨核细胞具有循环血小板中发现的环氧合酶和脂氧合酶活性的完整补充。
Guinea pig megakaryocytes were isolated to 75–90% purity, incubated with 1-14C-arachidonic acid and radioactive metabolites analyzed by thin layer chromatography. Thromboxane B2, 12-hydroxyheptadecatrienoic acid (HHT), and 12-hydroxyeicosatetraenoic acid (12-HETE) were the major products identified. Pre-incubation with indomethacin inhibited production of thromboxane B2and HHT. Comparison of metabolites produced by megakaryocytes and platelets from the same animals indicated that one megakaryocyte produced 2500–4000 times as much thromboxane B2, HHT, or 12-HETE as did a single platelet. These results suggest that isolated megakaryocytes possess the full complement of cyclooxygenase and lipoxygenase activities found in circulating platelets.