Prolactin enhancement of its own uptake at the choroid plexus.
Prolactin enhancement of its own uptake at the choroid plexus.
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DOI：
10.1210/endo.131.2.1639017
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发表时间：
1992-08
期刊：
影响因子：
4.8
通讯作者：
L. Mangurian;R. Walsh;B. Posner
中科院分区：
文献类型：
--
作者：
L. Mangurian;R. Walsh;B. Posner
脉络丛含有PRL受体，部分功能是将PRL从血液输送到脑脊液(CSF)。雌性大鼠每日三次注射氟哌啶醇(慢性高催乳素血症)，或死前4h注射溴隐亭或不注射溴隐亭，或单独注射溴隐亭4h(急性低催乳素血症)，或(3)一次血管注射绵羊PRL(急性高催乳素血症)，均可改变血PRL浓度。注射放射性标记的PRL后，通过体内放射自显影定量评估脉络丛摄取PRL的变化。血管注射放射性标记PRL后，通过对脑脊液标本进行十二烷基硫酸钠-聚丙烯酰胺凝胶电泳法，评估脉络丛摄取PRL的变化与PRL从血液到脑脊液转运的变化的相关性。放射自显影显示，与未经治疗的对照组动物相比，慢性和急性高催乳素血症均导致脉络丛摄取放射性标记的PRL显著增加。相反，与对照(未治疗)动物相比，溴隐亭对脉络丛催乳素的摄取没有影响。与未经治疗的对照组相比，慢性高催乳素血症，而不是急性高催乳素血症，导致放射性标记的PRL从血液到脑脊液的转运显著增加。这一结果与PRL循环上调脉络丛中PRL受体，从而增加PRL从血液到脑脊液的转运是一致的。
The choroid plexus contains PRL receptors that function in part to transport PRL from the blood into the cerebrospinal fluid (CSF). The blood PRL concentration of female rats was altered by 1) three daily injections of haloperidol (chronic hyperprolactinemia) with or without bromocriptine administration 4 h before death, 2) bromocriptine alone for 4 h (acute hypoprolactinemia), and 3) a single vascular injection of ovine PRL (acute hyperprolactinemia). Changes in the uptake of PRL by the choroid plexus was assessed by quantitative in vivo autoradiography after the injection of radiolabeled PRL. Correlation of changes in PRL uptake at the choroid plexus with changes in PRL transport from blood to CSF was evaluated by subjecting CSF samples to sodium dodecyl sulfate-polyacrylamide gel electrophoresis after vascular injection of radiolabeled PRL. Autoradiography revealed that both chronic and acute hyperprolactinemia resulted in a significant increase in the uptake of radiolabeled PRL by the choroid plexus compared to that in untreated control animals. In contrast, bromocriptine had no effect on PRL uptake at the choroid plexus relative to that in control (untreated) animals. Chronic hyperprolactinemia, but not acute hyperprolactinemia, resulted in a significant increase in the transport of radiolabeled PRL from the blood to the CSF compared to that in untreated controls. The results are consistent with the up-regulation of PRL receptors in the choroid plexus by circulating PRL and the consequent augmentation of transport of PRL from blood to CSF.