Delivery of MicroRNA-10b with Polylysine Nanoparticles for Inhibition of Breast Cancer Cell Wound Healing.
Delivery of MicroRNA-10b with Polylysine Nanoparticles for Inhibition of Breast Cancer Cell Wound Healing.
复制标题
DOI：
10.4137/bcbcr.s8513
复制
发表时间：
2012
期刊：
影响因子：
--
通讯作者：
Lei C
中科院分区：
文献类型：
--
作者：
Jin H;Yu Y;Chrisler WB;Xiong Y;Hu D;Lei C
最近的研究表明，微小RNA-10b（mir-10b）在转移性乳腺癌细胞中高表达，并通过抑制HOXD10靶点合成来正向调节乳腺癌细胞的迁移和侵袭。在这项研究中，我们设计了抗 mir-10b 分子，并将其与聚 L-赖氨酸 (PLL) 结合以测试递送有效性。在人乳腺细胞系 MDA-MB-231 的伤口愈合试验中，与成熟 mir-10b 小反义序列完全匹配的 RNA 分子序列与 PLL 混合时显示出强烈的抑制作用。由此产生的 PLL-RNA 纳米粒子以浓度依赖性方式将抗 microRNA 分子递送到乳腺癌细胞的细胞质中，并显示出可持续的有效性。
Recent studies revealed that micro RNA-10b (mir-10b) is highly expressed in metastatic breast cancer cells and positively regulates breast cancer cell migration and invasion through inhibition of HOXD10 target synthesis. In this study we designed anti-mir-10b molecules and combined them with poly L-lysine (PLL) to test the delivery effectiveness. An RNA molecule sequence exactly matching the mature mir-10b minor antisense showed strong inhibition when mixed with PLL in a wound-healing assay with human breast cell line MDA-MB-231. The resulting PLL-RNA nanoparticles delivered the anti-microRNA molecules into cytoplasm of breast cancer cells in a concentration-dependent manner that displayed sustainable effectiveness.