EFFECT OF OXYTOCIN ON TESTOSTERONE PRODUCTION BY ISOLATED RAT LEYDIG-CELLS IS MEDIATED VIA A SPECIFIC OXYTOCIN RECEPTOR
EFFECT OF OXYTOCIN ON TESTOSTERONE PRODUCTION BY ISOLATED RAT LEYDIG-CELLS IS MEDIATED VIA A SPECIFIC OXYTOCIN RECEPTOR
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DOI：
10.1095/biolreprod52.6.1268
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发表时间：
1995-06-01
影响因子：
3.6
通讯作者：
NICHOLSON, HD
中科院分区：
文献类型：
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作者：
FRAYNE, J;NICHOLSON, HD
用分离的成年大鼠睾丸间质细胞短期培养(<；10h)的方法，重新评价神经垂体激素催产素和精氨酸加压素(AVP)对睾丸类固醇生成的影响。10(-9)、10(-7)和10(-5)M浓度的催产素以剂量依赖的方式显著增加基础睾酮的产生，但对促黄体生成素刺激的睾酮的产生没有影响。用特异性催产素受体拮抗剂(OTA)测定其作用的特异性。在10(-9)~10(-5)M浓度范围内，OTA抑制催产素刺激的睾酮生成增加。此外，催产素激动剂Thr(4)Gly(7)催产素也引起基础睾酮产生的剂量依赖性增加。相反，从10(-9)到10(-5)M的AVP浓度对分离的间质细胞的基础睾酮产生没有持续的影响，但显著减少了促黄体生成素刺激的睾酮产生。将10(-7)和10(-5)M的OTA与10(-7)M的AVP结合，并不改变AVP的抑制作用。这些数据表明，催产素和AVP对体外培养的间质细胞产生睾酮有不同的影响，支持催产素通过特定的催产素受体在睾丸中发挥作用的假说。
The effect of the neurohypophysial hormones oxytocin and arginine vasopressin (AVP) on testicular steroidogenesis was reevaluated by use of short-term (< 10 h) cultures of isolated adult rat Leydig cells. Oxytocin at 10(-9), 10(-7), and 10(-5) M concentrations significantly increased basal testosterone production in a dose-dependent manner but had no effect on LH-stimulated testosterone production. The specificity of the effect was determined by use of the specific oxytocin receptor antagonist (OTA). OTA from 10(-9) to 10(-5) M concentrations inhibited the oxytocin-stimulated increase in testosterone production. Furthermore, the oxytocin agonist Thr(4) Gly(7) oxytocin also induced a dose-dependent increase in basal testosterone production. In contrast, AVP from 10(-9) to 10(-5) M concentrations did not consistently affect basal testosterone production by isolated Leydig cells, but significantly decreased LH-stimulated testosterone production. Inclusion of 10(-7) and 10(-5) M OTA with 10(-7) M AVP did not alter the inhibitory effect of the AVP.These data show that oxytocin and AVP have different effects on testosterone production by Leydig cells in vitro and support the hypothesis that oxytocin acts in the testis through a specific oxytocin receptor.