Angiotensin-induced variations of receptors in rat uterine membranes.
Angiotensin-induced variations of receptors in rat uterine membranes.
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血管紧张素诱导的大鼠子宫膜受体变化。
DOI：
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发表时间：
1975
期刊：
影响因子：
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通讯作者：
M. Worcel
中科院分区：
文献类型：
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作者：
E. Chevillotte;M. Devynck;F. Fyhrquist;P. Meyer;B. Rouzaire‐Dubois;M. Worcel
1.~3H标记的血管紧张素-II与大鼠子宫平滑肌细胞质膜特异性结合。证明了两类不同的结合部位对激素的亲和力不同。高亲和力结合部位(Kd29℃，约2.0×10(-8)mol/L)可能对应于参与生物反应的受体。2.双侧肾切除显著增加~3H标记的血管紧张素结合部位的浓度，这种现象似乎与继发于血浆血管紧张素抑制的受体部位的释放无关。这一现象可能与无肾大鼠子宫对血管紧张素的特异性超敏反应有关。3.肾切除大鼠输注血管紧张素II可降低~3H标记的血管紧张素结合位点数。4.提示血管紧张素受体浓度受循环血管紧张素浓度的调节。
1. 3H-labelled angiotensin II specfically binds to plasma membranes of rat uterine smooth muscle cells. Two classes of binding sites differing in their affinity for the hormone were demonstrated. The high-affinity binding sites (KD 29 degrees C approximately 2.0 X 10(-8) mol/l) probably correspond to the receptors involved in the biological response. 2. Bilateral nephrectomy significantly increases the concentration of 3H-labelled angiotensin-binding sites, a phenomenon which seems unrelated to the freeing of receptor sites secondary to the suppression of plasma angiotensin. This phenomenon may be responsible for the specific hypersensitivity in vitro to angiotensin of uteri excised in anephric rats as compared with normal rats. 3. Angiotensin II infusion in nephrectomized rats reduced the concentration of 3H-labelled angiotensin-binding sites. 4. It is suggested that the angiotensin receptor concentration is regulated by the concentration of circulating angiotensin.