An Identification and Expression Analysis of the ABCG Genes Related to Benzaldehyde Transportation among Three Prunus Species
An Identification and Expression Analysis of the ABCG Genes Related to Benzaldehyde Transportation among Three Prunus Species
复制标题
三种李树苯甲醛转运相关ABCG基因的鉴定及表达分析
DOI：
10.3390/horticulturae8060475
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发表时间：
2022-06-01
期刊：
影响因子：
3.1
通讯作者：
Yang, Shuting
中科院分区：
文献类型：
--
作者：
Hao, Ruijie;Chang, Jun;Yang, Shuting
李属植物多在早春开花，不同种类的花具有各自的花香特征，尤其是梅花在开花期能向空气中挥发出大量的苯类化合物。为了阐明梅花香气物质挥发性差异的分子基础，研究了梅花、杏和桃的内源和顶空挥发性成分以及ABCG基因的表达。采用气相色谱-质谱联用技术（GC-MS）对三种植物的花成分进行了分析，发现苯甲醛是其中的主要成分。同时，苯甲醛在梅、杏中的挥发效率远高于桃。此外，从梅、杏和桃中分别鉴定出130、135和133个ABC家族成员。WGCNA分析表明，候选ABCG基因与苯甲醛挥发效率呈正相关。荧光定量PCR结果表明ABCG 17更可能参与苯甲醛的跨膜转运。本研究旨在为阐明苯甲醛的跨膜转运机制提供理论依据，并为李属芳香花卉育种提供有价值的信息。
The plants of Prunus mostly bloom in early spring, and the flowers of various species possess their individual floral scent characteristics; Prunus mume, especially, can volatilize a large amount of benzenoid compounds into the air during the flowering phase. In order to elucidate the molecular basis of the differences in the volatile capacity of aromatic substances among Prunus flowers, the endogenous and the headspace volatile components and the expression of ABCG genes were studied among P. mume, P. armeniaca, and P. persica. We detected the floral components in the three species by gas chromatography-mass spectrometry (GC-MS), and we found that benzaldehyde was the key component. Meanwhile, the volatilization efficiency of benzaldehyde in P. mume and P. armeniaca were much higher than that in P. persica. Furthermore, 130, 135, and 133 ABC family members from P. mume, P. armeniaca, and P. persica were identified, respectively. WGCNA analysis demonstrated that candidate ABCG genes were positively correlated with benzaldehyde volatilization efficiency. Moreover, quantitative Real-time PCR indicated that ABCG17 was more likely to be involved in the transmembrane transport of benzaldehyde. This study aimed to provide a theoretical basis for elucidating the transmembrane transport of benzaldehyde and to further the valuable information for fragrant flower breeding in Prunus.