Molecular identification of fungal isolates and hatching success of green turtle (Chelonia mydas) nests
Molecular identification of fungal isolates and hatching success of green turtle (Chelonia mydas) nests
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DOI：
10.1007/s00203-018-1496-0
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发表时间：
2018-08-01
影响因子：
2.8
通讯作者：
Candan, Esra Deniz
中科院分区：
文献类型：
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作者：
Candan, Esra Deniz
本研究的目的是调查绿海龟巢的真菌多样性，并检查这些分离株之间的系统发育关系。在筑巢季节，从Sugozu海滩25%的幸存巢穴中收集了巢内沙子和失败的蛋的样本，Sugozu海滩是地中海濒危绿海龟最重要的筑巢海滩之一。用分子技术鉴定了23种真菌。分离真菌属曲霉属、青霉属、根霉属、放线菌属和棘霉属，其中2种未描述。本研究首次在所有的海龟巢中检出变色曲霉、四叉曲霉、管曲霉、米根霉、秀丽放线菌和变异棘霉。结果表明，36.4%的燕窝有真菌污染。受真菌污染的巢孵化成功率显著低于未受真菌污染的巢（P = 0.029）。此外，这可能对海龟构成威胁，并对保护工作者的健康构成威胁。本研究是首个与地中海东部海岸的海龟巢相关的分子系统发育研究，并为首次分离物种的真菌入侵海龟巢的文献做出了贡献。这些发现对改善巢地的潜在保护措施具有重要意义。
The aim of this study is to investigate the fungal diversity of green turtle nests and to examine phylogenetic relationships among these isolates. During the nesting season, samples of intra-nest sand and failed eggs were collected from 25% of the surviving nests in Sugozu Beaches, which are amongst the most important nesting beaches for endangered green turtles in the Mediterranean. Twenty-three fungi were identified by molecular techniques. Fungal isolates belonged to genera Aspergillus, Emericella, Rhizopus, Actinomucor and Apophysomyces with two undescribed species. Aspergillus variecolor, Aspergillus quadrilinieatus, Aspergillus tubingensis, Rhizopus oryzae, Actinomucor elegans and Apophysomyces variabilis were firstly detected in all sea turtle nests within this study. Our results demonstrate that 36.4% of the nests had fungal contamination. Also hatching success of the nests contaminated by fungi were significantly lower than the uncontaminated nests (P = 0.029). Also, this may represent a threat to marine turtles and a risk for the health of conservation workers. This study is the first molecular phylogenetic study associated with sea turtle nests in the eastern Mediterranean coast and contributes to the wider body of literature on fungal invasion of sea turtle nests with firstly isolated species. These findings are important for improving potential conservation measures for the nest sites.