Characterization of hemolysin of Moraxella bovis using a hemolysis-neutralizing monoclonal antibody
Characterization of hemolysin of Moraxella bovis using a hemolysis-neutralizing monoclonal antibody
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DOI：
10.1128/iai.68.6.3469-3474.2000
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发表时间：
2000-06-01
影响因子：
3.1
通讯作者：
Hodgson, JL
中科院分区：
文献类型：
--
作者：
Billson, FM;Harbour, C;Hodgson, JL
使用来自溶血性牛莫拉氏菌菌株 UQV 148NF 的浓缩细菌培养物上清液来免疫小鼠并产生单克隆抗体 (MAb)，其中之一，MAb G3/D7，中和了牛莫拉氏菌的溶血活性，并通过蛋白质印迹分析识别了代表每种不同菌毛血清群的溶血性牛莫拉氏菌菌株中的 94-kDa 蛋白质，角膜上皮的暴露牛分枝杆菌溶血素的作用在绵羊红细胞制剂中得到进一步表征，具有结合步骤和裂解进行所需的 Ca2+，与 RTX 家族类似。细菌外毒素。体外裂解活性的中和是牛分枝杆菌抗原存在的证据，该抗原可能能够保护牛免于发生传染性牛角结膜炎。
A concentrated bacterial culture supernatant from the hemolytic Moraxella bovis strain UQV 148NF was used to immunize mice and generate monoclonal antibodies (MAbs), One, MAb G3/D7, neutralized the hemolytic activity of M. bovis and recognized a 94-kDa protein by Western blot analysis in hemolytic M. bovis strains representing each of the different fimbrial serogroups, Exposure of corneal epithelial cells to ill, bovis concentrated culture supernatants demonstrated a role for an exotoxin in the pathogenesis of infectious bovine keratoconjunctivitis, while neutralization of hemolytic and cytotoxic activities by MAb G3/D7 implies that these activities are related or have common epitopes, The action of M. bovis hemolysin was further characterized in sheep erythrocyte preparations with a binding step and Ca2+ required for lysis to proceed, similar to the RTX family of bacterial exotoxins. Neutralization of lytic activity in vitro is evidence for the presence of M. bovis antigens, which may be capable of protecting cattle from the development of infectious bovine keratoconjunctivitis.