In situ visualization of bronchial submucosal glands and their secretory response to acetylcholine.
In situ visualization of bronchial submucosal glands and their secretory response to acetylcholine.
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支气管粘膜下腺体的原位可视化及其对乙酰胆碱的分泌反应。
DOI：
10.1152/ajplung.1997.272.2.l203
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发表时间：
1997
期刊：
影响因子：
--
通讯作者：
Ballard,ST
中科院分区：
文献类型：
--
作者：
Inglis,SK;Corboz,MR;Taylor,AE;Ballard,ST
气道粘膜下腺分泌大分子和液体，但这些物质的分泌机制尚不清楚。在本研究中，使用视频显微镜直接观察离体猪远端支气管粘膜下腺，并观察它们对乙酰胆碱（一种腺体分泌剂）的反应。粘膜下腺根据其末端收集管的形态分为“窦状”、“线状”或“卷状”腺。由于胃窦管腺最容易被观察到，因此我们详细研究了胃窦管腺对乙酰胆碱的反应。在添加10微米ACh后5-10秒内，腺管开口与气道表面的横截面积增加了数倍，但在1分钟内恢复到ACh前的尺寸。添加ACh后30 s ~ 10 min，球形颗粒（1 ~ 10微米）从远端腺泡进入窦管，并通过窦管开口到达气道表面。一些微粒被保留在肛管内，在那里它们通过存在于肛管内的纤毛的作用保持恒定的运动。这些颗粒可能含有黏液和/或浆液细胞的大分子分泌产物，它们在腺体导管内保持球形，表明分泌产物是膜结合的。据我们所知，这些研究提供了气道粘膜下腺分泌的原位观察的第一个描述。
Airway submucosal glands secrete both macromolecules and liquid, yet the mechanisms by which these substances are secreted are not well understood. In this study, a video microscope was used to directly visualize the submucosal glands in isolated porcine distal bronchi and to observe their responses to acetylcholine (ACh), a glandular secretagogue. Submucosal glands were classified as either "antral," "linear," or "convoluted" glands based on the morphology of their terminal collecting ducts. Because antral duct glands were most easily visualized, the response to ACh was studied in detail in this gland type. Within 5-10 s after addition of 10 microM ACh, the cross-sectional area of the gland duct openings to the airway surface increased severalfold but returned to pre-ACh dimensions within 1 min. Between 30 s and 10 min after ACh addition, spherical particles (1-10 microm) entered the antral ducts from distal acini and exited through the duct openings to the airway surface. Some of the particles were retained within the antral duct where they were kept in constant motion by the action of cilia present within the antral duct. The particles, which are likely to contain the macromolecular secretory products of mucous and/or serous cells, maintained their spherical shape within the gland duct, suggesting that the secretion product was membrane bound. To our knowledge, these studies provide the first description of airway submucosal gland secretion as viewed in situ.