Antifungal Activity of 2 Lactic Acid Bacteria of the Weissella Genus Isolated from Food
Antifungal Activity of 2 Lactic Acid Bacteria of the Weissella Genus Isolated from Food
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DOI：
10.1111/j.1750-3841.2011.02257.x
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发表时间：
2011-08-01
影响因子：
3.9
通讯作者：
Thonart, Philippe
中科院分区：
文献类型：
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作者：
Ndagano, Dora;Lamoureux, Thibaut;Thonart, Philippe
本研究对从面粉和发酵木薯中分离的116株乳酸菌（LAB）进行了抑菌活性筛选。从116株菌株中筛选出3株抑菌活性最强的菌株。这3株菌株分别为植物乳杆菌VE56、猪魏塞尔菌FMF4B16和副肠杆菌LC11。研究了其抗真菌活性的化合物。菌株在酸性ph下对目标霉菌具有抑制活性。然而，根据计算的最小抑制浓度（MIC），拒绝了有机酸的影响。在无细胞上清液中检测抗真菌化合物，并检测不同浓度的苯乳酸（PLA），植物Lb. plantarum VE56的最大浓度为0.56 mM。羟基脂肪酸，如2-羟基-4-甲基戊酸，也参与了植物芽孢杆菌VE56和副肠芽孢杆菌LC11的抑制活性。抗真菌LAB已知可产生聚乳酸和3-羟基脂肪酸以及其他具有抗真菌活性的有机酸。这篇简短的交流主要集中在魏塞拉属的抗真菌活性。抗真菌活性归因于抗真菌化合物，如聚乳酸、2-羟基-4-甲基戊酸和其他有机酸。然而，无细胞上清中产生的浓度太低，无法与它们的MIC相比，这表明抑制活性是由这些不同化合物的协同作用引起的。
In the present study, a total of 116 lactic acid bacteria (LAB) strains isolated from Mill flour and fermented cassava were screened for their antifungal activity. Three strains among 116 were selected for their strongest inhibitory activity against food molds. These 3 strains were Lactobacillus plantarum VE56, Weissella cibaria FMF4B16, and W. paramesenteroides LC11. The compounds responsible for the antifungal activity were investigated. The strains displayed an inhibitory activity against targeted molds at acidic pH. However, the influence of organic acids was rejected according to the calculated minimal inhibitory concentration (MIC). Antifungal compounds were investigated in the cell-free supernatants and phenyllactic acid (PLA) was detected in different amounts with a maximal concentration for Lb. plantarum VE56 (0.56 mM). Hydroxy fatty acid, such as 2-hydroxy-4-methylpentanoic acid, was also produced and involved in the inhibitory activity of Lb. plantarum VE56 and W. paramesenteroides LC11. Antifungal LAB are known to produce PLA and 3-hydroxy fatty acids and other organic acids with antifungal activity. This short communication focuses on antifungal activity from Weissella genus. The antifungal activity was attributed to antifungal compounds identified such as PLA, 2-hydroxy-4-methylpentanoic acid, and other organic acids. Nevertheless, the concentration produced in the cell-free supernatant was too low to compare to their MIC, suggesting that the inhibitory activity was caused by a synergy of these different compounds.