Estrogen receptor profiles across tissues from male and female Rattus norvegicus
Estrogen receptor profiles across tissues from male and female Rattus norvegicus
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DOI：
10.1186/s13293-019-0219-9
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发表时间：
2019-01-11
影响因子：
7.9
通讯作者：
Lindsey, Sarah H.
中科院分区：
文献类型：
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作者：
Hutson, Dillion D.;Gurrala, Rakesh;Lindsey, Sarah H.
背景雌激素由芳香酶 (CYP19A1) 形成，并通过三种已识别的受体 ER (ESR1)、ER ss (ESR2) 和 G 蛋白偶联雌激素受体 (GPER) 发出信号。了解每种受体对雌激素信号传导的相对贡献可能会阐明这种性激素在组织中的不同影响，并且 PCR 技术的最新发展允许对多个靶标进行绝对定量和直接比较。我们假设这种方法将揭示雌激素受体 mRNA 的组织和性别特异性差异。方法测量了 6 名男性和 6 名男性的 4 个心血管组织（心脏、主动脉、肾脏和肾上腺）、3 个脑区（体感皮层、海马和前额皮质）和生殖组织（卵巢、乳腺、子宫、睾丸）中的 ESR1、ESR2、GPER 和 CYP19A1。雌性成年 Sprague-Dawley 大鼠。结果 GPER mRNA 表达在两性的所有组织中相对稳定，范围为 5.49 至 113 拷贝/ng RNA，差异为 21 倍。相比之下，ESR1/ESR2 在不同组织之间存在差异，尽管在器官系统内相似。 ESR1 范围为 4.46 至 614 拷贝/ng RNA（138 倍差异），而 ESR2 范围为 0.154 至 83.1 拷贝/ng RNA（540 倍）。除肾 ESR1 外，广泛不存在显着的性别差异（女性 206 拷贝/ng RNA，男性 614 拷贝/ng RNA，P
BackgroundEstrogen is formed by the enzyme aromatase (CYP19A1) and signals via three identified receptors ER (ESR1), ER ss (ESR2), and the G protein-coupled estrogen receptor (GPER). Understanding the relative contribution of each receptor to estrogenic signaling may elucidate the disparate effects of this sex hormone across tissues, and recent developments in PCR technology allow absolute quantification and direct comparison of multiple targets. We hypothesized that this approach would reveal tissue- and sex-specific differences in estrogen receptor mRNA.MethodsESR1, ESR2, GPER, and CYP19A1 were measured in four cardiovascular tissues (heart, aorta, kidney, and adrenal gland), three brain areas (somatosensory cortex, hippocampus, and prefrontal cortex), and reproductive tissues (ovaries, mammary gland, uterus, testes) from six male and six female adult Sprague-Dawley rats.ResultsGPER mRNA expression was relatively stable across all tissues in both sexes, ranging from 5.49 to 113 copies/ng RNA, a 21-fold difference. In contrast, ESR1/ESR2 were variable across tissues although similar within an organ system. ESR1 ranged from 4.46 to 614 copies/ng RNA (138-fold difference) while ESR2 ranged from 0.154 to 83.1 copies/ng RNA (540-fold). Significant sex differences were broadly absent except for renal ESR1 (female 206 vs. male 614 copies/ng RNA, P