Influence of pore size and hydroxyapatite deposition in poly(L-lactide-co-glycolide) scaffolds on osteoblast-like cells cultured in static and dynamic conditions
Influence of pore size and hydroxyapatite deposition in poly(L-lactide-co-glycolide) scaffolds on osteoblast-like cells cultured in static and dynamic conditions
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DOI：
10.1016/j.matlet.2019.01.039
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发表时间：
2019-04-15
影响因子：
3
通讯作者：
Pamula, Elzbieta
中科院分区：
文献类型：
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作者：
Krok-Borkowicz, Malgorzata;Filova, Elena;Pamula, Elzbieta
本研究的目的是评估微观结构参数、羟基磷灰石（HA）的存在和细胞培养条件对聚（L-丙交酯-乙交酯）（PLGA）支架上骨细胞生长的影响。采用羟基磷灰石(HA)对孔隙率85%、孔径250-320μm和400-600μm的支架进行修饰。 MG-63 成骨细胞样细胞在这些支架上在静态和动态条件下培养直至第 14 天。HA 沉积物增加了改良支架的润湿性。直到第 14 天，所有支架类型在静态条件下的细胞粘附和增殖都具有可比性。在动态条件下，400-600HA 上的细胞生长速度最高，这与静态培养中的细胞生长相当。 250-320 和 400-600 未修饰支架上的细胞生长显着降低。对于250-320HA，细胞数量显着高于250-320，但低于400-600HA。因此，HA 在具有较大孔径（即 400-600HA）的 PLGA 支架上沉积，可增强动态条件下的细胞生长。在所有支架上，细胞产生成骨标记物骨桥蛋白和骨钙蛋白。最丰富的骨桥蛋白水平为400-600HA。 (C) 2019 Elsevier B.V. 保留所有权利。
The aim of this study was to assess the influence of microstructural parameters, the presence of hydroxyapatite (HA) and cell culture conditions on growth of bone cells on poly(L-lactide-co-glycolide) (PLGA) scaffolds. The scaffolds with 85% porosity and pore size: 250-320 mu m and 400-600 mu m were modified with hydroxyapatite (HA). MG-63 osteoblast-like cells were cultured in static and dynamic conditions on these scaffolds up to day 14. HA deposits increased wettability of the modified scaffolds. Cell adhesion and proliferation in static conditions were comparable for all scaffold types till day 14. In dynamic condition, the highest cell growth was on 400-600HA, which was comparable to that in static culture. Significantly lower cell growth was on 250-320 and 400-600 non-modified scaffolds. For 250-320HA cell number was significantly higher than on 250-320, but lower than on 400-600HA. Thus, deposition of HA on PLGA scaffolds with higher size of pores, i.e. 400-600HA, enhanced cell growth in dynamic conditions. On all scaffolds cells produced osteogenic markers osteopontin and osteocalcin. The most abundant osteopontin level was on 400-600HA. (C) 2019 Elsevier B.V. All rights reserved.