A gene from Alcaligenes denitrificans that confers albicidin resistance by reversible antibiotic binding.
A gene from Alcaligenes denitrificans that confers albicidin resistance by reversible antibiotic binding.
复制标题
来自脱氮产碱菌的基因，通过可逆的抗生素结合赋予 albicidin 抗性。
作者：
W. V. Basnayake;R. Birch
Albicidin 抗生素特异性阻断原核生物 DNA 复制。从脱氮产碱菌土壤分离株中克隆的 albicidin 抗性基因 (albB) 编码一种 23 kDa 的蛋白质，能够通过可逆结合使 albicidin 解毒。该机制在细胞内起作用，以保护表达克隆抗性基因的对 albicidin 敏感的大肠杆菌中的 DNA 复制，在周围介质中存在 1.5 微克 albicidin ml-1 的情况下，可以将其诱导五倍。 621 bp 的编码区与产酸克雷伯菌的 albicidin 抗性基因 (albA) 具有部分 DNA 序列同源性，但由于重排和移码导致蛋白质同源性丧失。尽管这两个基因使用不同的密码子来共享氨基酸，但脱氮放线菌和产酸克雷伯菌的 Albicidin 抗性 (Albr) 蛋白之间存在一小段 N 端同源性区域。 N 端同源性表明有一个共同的功能域；这通过缺失分析、翻译融合和合成寡肽与albicidin的结合得到证实。抗生素结合使大肠杆菌具有高水平的 albicidin 抗性。该基因似乎是转移到植物中的有用候选基因，以保护质体DNA复制免受与甘蔗叶烫病相关的albicidin植物毒素的抑制。
Albicidin antibiotics specifically block prokaryote DNA replication. The albicidin resistance gene (albB) cloned from a soil isolate of Alcaligenes denitrificans encodes a 23 kDa protein capable of detoxifying albicidin by reversible binding. This mechanism operates intracellularly to protect DNA replication in albicidin-sensitive Escherichia coli expressing the cloned resistance gene, which can be induced fivefold in the presence of 1.5 micrograms albicidin ml-1 in the surrounding medium. The coding region of 621 bp has regions with partial DNA sequence homology to an albicidin resistance gene (albA) from Klebsiella oxytoca, but with rearrangements and frame-shifts resulting in loss of protein homology. There is a short region of N-terminal homology between the albicidin resistance (Albr) proteins from A. denitrificans and K. oxytoca, although the two genes use different codons for shared amino acids. The N-terminal homology suggested a common functional domain; this was confirmed by deletion analysis, translational fusions and albicidin binding by a synthetic oligopeptide. Antibiotic binding provides a high level of albicidin resistance in E. coli. The gene appears to be a useful candidate for transfer to plants to protect plastid DNA replication from inhibition by albicidin phytotoxins involved in sugarcane leaf scald disease.
新的失控复制质粒克隆载体和新生霉素对失控复制的抑制。
影响因子：
3.5
作者：
Uhlin,BE;Schweickart,V;Clark,AJ
通讯作者：
Clark,AJ