Human saphenous vein organ culture: A useful model of intimal hyperplasia?
Human saphenous vein organ culture: A useful model of intimal hyperplasia?
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DOI：
10.1016/s1078-5884(96)80134-1
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发表时间：
1996-01-01
影响因子：
5.7
通讯作者：
London, NJM
中科院分区：
文献类型：
--
作者：
Porter, KE;Varty, K;London, NJM
目的：虽然细胞培养技术和内膜增生的动物模型增加了我们目前对静脉移植物狭窄病因的理解，但这些研究的结果很难与人类情况相关联。设计：本研究旨在通过比较培养静脉中发生的变化与病理性静脉移植狭窄中观察到的变化来验证人隐静脉的器官培养，并确定合适的材料和方法：将大隐静脉段培养14天，福尔马林固定，免疫组化染色。对新切除的狭窄进行固定和类似处理。一些细胞增殖的标志物进行了评估，以确定一个最适合于这个特定的model.Results：显着的相似性，观察到在细胞和细胞外基质的组合物，和电子显微镜显示，无论是培养的静脉和病理病变的新生内膜含有丰富的平滑肌细胞的分泌表型。溴脱氧尿苷被证明是最可靠的增殖标记物，并揭示了静脉内膜浅层的早期增殖引起新生内膜的形成。增殖和新生内膜厚度在培养第14天达到最大。此后增殖迅速下降，新生内膜maintenance.Conclusions：发生在培养的静脉和移植狭窄的变化有许多相似之处，从而证明使用器官培养作为一个有价值的实验工具。
Objectives: Although cell culture techniques and animal models of intimal hyperplasia have increased our current understanding of the aetiology of vein graft stenosis, the results of such studies have been difficult to relate to the human situation.Design: The present study was designed to validate an organ culture of human saphenous vein by comparing the changes occurring in cultured vein with those seen in pathological vein graft stenoses and to identify a suitable marker of cell proliferation.Materials and methods: Saphenous vein segments were cultured for 14 days, fixed in formalin and processed for immunohistochemistry. Freshly excised stenoses were fixed and processed similarly. A number of markers of cell proliferation were evaluated in the culture system in order to identify the one best suited to this particular model.Results: Marked similarities were observed in the cellular and extracellular matrix composition, and electron microscopy revealed that both the neointima of the cultured vein and the pathological lesion contained an abundance of smooth muscle cells of a secretory phenotype. Bromodeoxyuridine proved to be the most reliable proliferation marker and revealed that early proliferation in the superficial layers of the vein intima gave rise to the formation of neointima. Both proliferation and neointimal thickness were maximal by day 14 in culture. Proliferation declined rapidly thereafter and the neointima was maintained.Conclusions: The changes occurring in cultured vein and graft stenoses bore many similarities, thereby justifying the use of organ culture as a valuable experimental tool.