Green pyomelanin-mediated synthesis of gold nanoparticles: modelling and design, physico-chemical and biological characteristics
Green pyomelanin-mediated synthesis of gold nanoparticles: modelling and design, physico-chemical and biological characteristics
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DOI：
10.1186/s12934-019-1254-2
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发表时间：
2019-12-03
影响因子：
6.4
通讯作者：
Kus-Liskiewicz, Malgorzata
中科院分区：
文献类型：
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作者：
Ben Tahar, Imen;Fickers, Patrick;Kus-Liskiewicz, Malgorzata
背景资料：纳米粒子的合成及其在材料中的结合是化学、物理和材料科学中研究最多的课题之一。金纳米颗粒由于其抗菌和抗癌活性而在医学、生物医学成像和诊断测试中具有应用。尽管纳米颗粒的化学合成得到了很好的表征和控制，但它们依赖于苛刻的化学条件和有机溶剂的利用，并产生有毒残留物。因此，最近已经开发了用于NPs合成的更绿色和更可持续的替代方法。这些方法使用微生物，主要是酵母或酵母细胞提取物。用培养物上清液合成NP大多数时候是优选的方法，因为它有利于回收NP的纯化方案。在细胞或细胞壁内形成的NP的提取是费力的、耗时的并且不具有成本效益。纳米粒子的生物活性，即抗菌和抗癌，是已知的有关纳米粒子的形状，大小和大小distributions.Results：在这里，我们报道了绿色合成金纳米粒子（AuNPs）介导的脓黑素从酵母解脂耶氏酵母纯化。使用三水平四因子Box-Behnken设计（BBD）来评估温度、pH、金盐和脓黑素浓度对纳米颗粒尺寸分布的影响。基于BBD，建立了二次模型，并应用于预测产生具有特定尺寸的AuNPs的实验参数。合成的纳米颗粒的中值粒径为104 nm，是纳米晶结构，主要是多边形或球形。它们表现出高的胶体稳定性，zeta电位为-28.96 mV，中等的多分散指数为0.267。在两种哺乳动物细胞系，即小鼠成纤维细胞（NIH 3 T3）和人骨肉瘤细胞（U2 OS）上研究了AuNP的细胞毒性的缺乏。细胞活力仅在AuNP浓度高于160 μ g/mL时降低。此外，他们并没有影响上的细胞morphology.Conclusion：我们的研究结果表明，不同的工艺参数显着影响纳米颗粒的大小，但与数学模型，它是可能的，以定义的大小AuNPs。此外，这种基于黑色素的金纳米颗粒既没有细胞毒性作用，也没有改变细胞形态。
Background: Synthesis of nanoparticles (NPs) and their incorporation in materials are amongst the most studied topics in chemistry, physics and material science. Gold NPs have applications in medicine due to their antibacterial and anticancer activities, in biomedical imaging and diagnostic test. Despite chemical synthesis of NPs are well characterized and controlled, they rely on the utilization of harsh chemical conditions and organic solvent and generate toxic residues. Therefore, greener and more sustainable alternative methods for NPs synthesis have been developed recently. These methods use microorganisms, mainly yeast or yeast cell extract. NPs synthesis with culture supernatants are most of the time the preferred method since it facilitates the purification scheme for the recovery of the NPs. Extraction of NPs, formed within the cells or cell-wall, is laborious, time-consuming and are not cost effective. The bioactivities of NPs, namely antimicrobial and anticancer, are known to be related to NPs shape, size and size distribution.Results: Herein, we reported on the green synthesis of gold nanoparticles (AuNPs) mediated by pyomelanin purified from the yeast Yarrowia lipolytica. A three levels four factorial Box-Behnken Design (BBD) was used to evaluate the influence of temperature, pH, gold salt and pyomelanin concentration on the nanoparticle size distribution. Based on the BBD, a quadratic model was established and was applied to predict the experimental parameters that yield to AuNPs with specific size. The synthesized nanoparticles with median size value of 104 nm were of nanocrystalline structure, mostly polygonal or spherical. They exhibited a high colloidal stability with zeta potential of - 28.96 mV and a moderate polydispersity index of 0.267. The absence of cytotoxicity of the AuNPs was investigated on two mammalian cell lines, namely mouse fibroblasts (NIH3T3) and human osteosarcoma cells (U2OS). Cell viability was only reduced at AuNPs concentration higher than 160 mu g/mL. Moreover, they did not affect on the cell morphology.Conclusion: Our results indicate that different process parameters affect significantly nanoparticles size however with the mathematical model it is possible to define the size of AuNPs. Moreover, this melanin-based gold nanoparticles showed neither cytotoxicity effect nor altered cell morphology.