Transcriptome -wide modulation combined with morpho-physiological analyses of Typha orientalis roots in response to lead challenge.
Transcriptome -wide modulation combined with morpho-physiological analyses of Typha orientalis roots in response to lead challenge.
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DOI：
10.1016/j.jhazmat.2019.121405
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发表时间：
2020-02
影响因子：
13.6
通讯作者：
Xiaoying Xu;Qi Chen;Shuangrong Mo;Ying Qian;Xiaoxia Wu;Yiqiao Jin;H. Ding
中科院分区：
文献类型：
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作者：
Xiaoying Xu;Qi Chen;Shuangrong Mo;Ying Qian;Xiaoxia Wu;Yiqiao Jin;H. Ding
铅（Pb）是一种常见的环境污染物，香蒲（TyphaorientalisPresl）是一种大型挺水植物，具有耐铅和富集铅的功能，但目前有关香蒲耐铅的分子生物学研究较少。orientalistowards铅是已知的。本文研究了不同浓度铅胁迫下植物对铅的积累规律及影响铅胁迫响应的主要因素。铅主要积累在根中，并主要分布在细胞壁和膜系统中。通过RNA-seq分析鉴定了铅暴露后侧柏根中的差异表达基因（DEG）。在0.10 mM和0.25 mM Pb 2+处理组中，相对于对照，共检测到3275个DEG。这些基因中的许多与氧化还原过程，金属转运，蛋白激酶/磷酸化，和DNA结合转录因子，这被证明是铅响应DEG。映射京都基因和基因组百科全书（KEGG）数据库，“苯丙素类生物合成”被分析为0.10 mM和0.25 mM Pb 2+处理的重叠DEG的重要模块的主要途径。此外，一个功能未知的先导反应基因ToLR 1引起了人们的兴趣。利用cDNA末端快速扩增（RACE）技术克隆了ToLR 1基因的全长序列，并在拟南芥（Arabidopsis thaliana）中进行了过量表达，获得了对Pb胁迫抗性增强的ToLR 1基因。这是第一个提供详细的基因组信息的铅响应基因在东方锥虫。此外，这项研究提供了新的见解的分子机制的反应ofT。orientalisandotherapy对Pb胁迫的响应。本研究中确定的关键基因可能作为潜在的目标基因工程植物修复。
Lead (Pb) is a common pollutant in many environments, including in the soil, water, and/or air.Typha orientalisPresl, a large emergent aquatic plant, has been reported to function as a Pb-tolerant and Pb-accumulating plant; however, very little molecular information regarding the tolerance ofT. orientalistowards Pb is known. In this study, Pb accumulation and key factors involved in the Pb stress response at different Pb concentrations were investigated. Pb was primarily accumulated in the roots and was mainly located in the cell wall and membrane systems. Differentially expressed genes (DEGs) were identified inT. orientalisroots after Pb exposure via RNA-seq analyses. In the 0.10 mM and 0.25 mM Pb2+-treated groups, a total of 3275 DEGs were detected relative to the control. Many of these genes were associated with oxidation-reduction processes, metal transport, protein kinase/phosphorylation, and DNA binding transcription factors, which were shown to be Pb-responsive DEGs. Mapping Kyoto Encyclopedia of Genes and Genomes (KEGG) database, “phenylpropanoid biosynthesis” was analyzed as the major pathway of the important modules of overlapping DEGs of 0.10 mM and 0.25 mM Pb2+treatments. Furthermore, a lead response gene namedToLR1with unknown function was of particular interest. The full-length ofToLR1sequence was cloned using rapid amplification of cDNA ends (RACE) and overexpressed inArabidopsis thaliana, which resulted in enhanced resistance to Pb stress. This is the first report providing genomic information detailing Pb responsive genes inT. orientalis. Moreover, this study provides novel insights into the molecular mechanisms underlying the response ofT. orientalisand other accumulators towards Pb stress. The key genes identified in this study may serve as potential targets for genetic engineering targeting phytoremediation.