Study of plasma metabolic profiling and biomarkers of chronic unpredictable mild stress rats based on gas chromatography/mass spectrometry
Study of plasma metabolic profiling and biomarkers of chronic unpredictable mild stress rats based on gas chromatography/mass spectrometry
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基于气相色谱/质谱法的慢性不可预见轻度应激大鼠血浆代谢谱及生物标志物研究
DOI：
10.1002/rcm.4809
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发表时间：
2010-12-01
影响因子：
2
通讯作者：
Qin, Xue-Mei
中科院分区：
文献类型：
--
作者：
Li, Zhen-Yu;Zheng, Xing-Yu;Qin, Xue-Mei
对慢性不可预测的轻度应激（CUMS）大鼠进行了代谢组学研究。使用气相色谱/质谱分析从经 CUMS 处理的 Sprague-Dawley (SD) 大鼠获得的血浆。在检测到的化合物中鉴定出 37 种代谢物。随后使用 t 检验和主成分分析 (PCA) 进行的数据分析揭示了 CUMS 治疗后大鼠血浆中显着的代谢变化。模型组和对照组之间实现了明显的分离，并且对照组和CUMS组的血浆样本中氨基酸、糖、有机酸和脂肪酸等12种代谢物的水平存在显着差异。这些观察结果表明，抑郁状态可能与氨基酸代谢、能量代谢和糖代谢的扰动有关。该研究表明，代谢组学方法可以作为潜在的强大工具来研究某些病理生理状况（例如抑郁症）的生化变化，作为早期诊断手段。版权所有 (C) 2010 约翰·威利父子有限公司
A metabolomic investigation of chronic unpredictable mild stress (CUMS) rats was carried out. Plasma obtained from Sprague-Dawley (SD) rats treated by CUMS was analyzed using gas chromatography/mass spectrometry. Thirty-seven metabolites were identified among the detected compounds. Subsequent data analysis using the t test and principal component analysis (PCA) revealed significant metabolic changes in the rats' plasma after CUMS treatment. Clear separation between the model and control group was achieved, and the level of twelve metabolites, including amino acids, sugar, organic acids and fatty acids, were significantly different between plasma samples from the controls and CUMS group. These observations suggested that the depressed state may be associated with perturbation of amino acid metabolism, energy metabolism and glycometabolism. The study suggested that the metabolomics approach could be used as a potential powerful tool to investigate the biochemical change in certain physiopathological conditions, such as depression, as an early diagnostic means. Copyright (C) 2010 John Wiley & Sons, Ltd.