Genome-wide analysis, expression dynamics and varietal comparison of NAC gene family at various developmental stages in Morus notabilis
Genome-wide analysis, expression dynamics and varietal comparison of NAC gene family at various developmental stages in Morus notabilis
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DOI：
10.1007/s00438-016-1186-z
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发表时间：
2016-06-01
影响因子：
3.1
通讯作者：
Khurana, Paramjit
中科院分区：
文献类型：
--
作者：
Baranwal, Vinay Kumar;Khurana, Paramjit
NAC基因是植物中重要的转录因子，在植物中形成了一个大家族。它们在生长和发育中发挥重要作用，并参与调节应激反应基因。在本研究中，最近公布的桑葚基因组中的NAC基因库已被确定，其中包括总共79个成员。结构分析表明，桑葚NAC基因大多含有两个内含子。由它们编码的蛋白质显示出广泛的等电点，这表明它们在不同的微细胞环境中具有不同的作用。系统发育和保守基序分析阐明了这些基因的15个亚组的存在，沿着两个新的亚组具有不同的保守基序，这是不存在于拟南芥。基因本体论术语富集分析和顺式元件鉴定，从他们假定的1 K上游调控区表明，他们可能在重要的生物过程，如器官形成，分生组织的建立，衰老，以及各种生物和非生物胁迫的作用。在不同发育阶段的表达分析导致其在不同组织中的优先表达的鉴定。本研究为桑葚NAC基因家族的结构、功能、表达和进化提供了坚实的背景信息。
NAC genes are important transcription factors and forms a large family in plants. They have shown to play an important role in growth and development and have also been shown to involve in regulation of stress-responsive genes. In the present study, a repertoire of NAC genes in recently published mulberry genome has been identified which consists of a total of 79 members. Structural analysis revealed that most of the NAC genes in mulberry contain two introns. The proteins encoded by them show a wide range of isoelectric points suggestive of their varied roles in varying microcellular environment. Phylogenetic and conserved motif analysis elucidate the presence of 15 sub-groups of these genes along with two novel sub-groups having distinct conserved motifs which are not present in Arabidopsis. Gene ontology term enrichment analysis and cis-element identification from their putative 1 K upstream regulatory region indicates their possible role in important biological processes like organ formation, meristem establishment, senescence, and various biotic and abiotic stresses. Expression analysis across various developmental stages led to identification of their preferential expression in diverse tissues. Taken together, this work provides a solid background information related to structure, function, expression and evolution of NAC gene family in mulberry.