Effects of three permeases on arginine utilization in Saccharomyces cerevisiae.
Effects of three permeases on arginine utilization in Saccharomyces cerevisiae.
复制标题
三种渗透酶对酿酒酵母精氨酸利用的影响
DOI：
10.1038/srep20910
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发表时间：
2016-02-11
影响因子：
4.6
通讯作者：
Zhou J
中科院分区：
文献类型：
--
作者：
Zhang P;Du G;Zou H;Chen J;Xie G;Shi Z;Zhou J
精氨酸在细胞功能和新陈代谢中发挥着重要作用。精氨酸的摄取主要通过三种氨基酸渗透酶 Alp1p、Gap1p 和 Can1p 发生，它们既充当氨基酸利用的转运蛋白又充当受体。在这项研究中，构建了七个具有抑制精氨酸利用的通透酶缺陷的不同组合的突变体。测量了它们对精氨酸代谢的影响。这三种氨基酸通透酶在野生型 (WT)、Δalp1Δgap1Δcan1 和 Δnpr1 菌株中也分别过表达。当精氨酸为唯一氮源时，在 YNB 培养基中，Δcan1、Δgap1Δcan1 和 Δalp1Δgap1Δcan1 突变体的生长和精氨酸利用受到抑制。同时，Alp1p 和 Can1p 的过度表达增强了 WT、Δalp1Δgap1Δcan1 和 Δnpr1 的生长和精氨酸利用。此外，与Δalp1Δgap1Δcan1中的Alp1p相比，Can1p的过度表达导致OD600增加26.7%，精氨酸利用率增加29.3%。转录分析表明，三种氨基酸通透酶对精氨酸利用和相关基因调控的影响与其个体特征密切相关。然而，对于不同的突变体组合，它们的总体效果是不同的。这里提出的结果表明不同氨基酸通透酶对氨基酸利用和代谢的调节可能具有一些协同作用。
Arginine plays an important role in cellular function and metabolism. Arginine uptake mainly occurs through three amino acid permeases, Alp1p, Gap1p and Can1p, which act as both transporters and receptors for amino acid utilization. In this study, seven mutants were constructed with different combinations of permease deficiencies that inhibit arginine utilization. Their effects on arginine metabolism were measured. The three amino acid permeases were also individually overexpressed in wild-type (WT), Δalp1Δgap1Δcan1 and Δnpr1 strains. The growth and arginine utilization of Δcan1, Δgap1Δcan1 and Δalp1Δgap1Δcan1 mutants were suppressed in YNB medium when arginine was the sole nitrogen source. Meanwhile, overexpression of Alp1p and Can1p enhanced growth and arginine utilization in WT, Δalp1Δgap1Δcan1 and Δnpr1. Besides, overexpression of Can1p caused a 26.7% increase in OD600 and 29.3% increase in arginine utilization compared to that of Alp1p in Δalp1Δgap1Δcan1. Transcription analysis showed that the effects of three amino acid permeases on the arginine utilization and the regulation of related genes, were tightly related to their individual characteristics. However, their overall effects were different for different combinations of mutants. The results presented here suggest some possible synergistic effects of different amino acid permeases on regulation of amino acid utilization and metabolism.