Expression profile analysis in multiple human tumors identifies L1 (CD171) as a molecular marker for differential diagnosis and targeted therapy
Expression profile analysis in multiple human tumors identifies L1 (CD171) as a molecular marker for differential diagnosis and targeted therapy
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DOI：
10.1016/j.humpath.2006.03.014
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发表时间：
2006-08-01
期刊：
影响因子：
3.3
通讯作者：
Fogel, Mina
中科院分区：
文献类型：
--
作者：
Huszar, Monica;Moldenhauer, Gerhard;Fogel, Mina
L1细胞粘附分子（CD171）是卵巢癌和子宫内膜癌的一个非常不利的预后生物标志物。在这里，我们使用单克隆抗体L1- 11a和新型单克隆抗体L1-14.10对正常成人和广泛的良性和恶性肿瘤中的L1表达进行了免疫组织化学调查。在正常组织中，L1在成人组织和儿童肾脏的集合小管以及周围神经束中表达。在女性生殖道肿瘤中，除了卵巢癌和子宫内膜癌外，在子宫颈和输卵管腺癌中也检测到l1。非肿瘤性表达L1的肿瘤包括恶性黑色素瘤、嗜铬粒蛋白阳性的结肠癌、膀胱透明细胞腺癌、嗜铬细胞瘤、小细胞肺癌和神经系统肿瘤。L1在乳腺癌、胃肠道癌、胃肠道类癌、肾透明细胞癌、前列腺腺癌和间皮瘤中缺失。令人惊讶的是，L1在已建立的乳腺癌和肾癌细胞系中的表达并不能预测其在这些人类肿瘤中的存在。我们的研究结果表明，L1在肿瘤中的表达并非普遍存在，而仅限于某些亚型，可能是鉴别诊断和基于抗体的治疗的有用分子标记。(c) 2006爱思唯尔公司版权所有。
L1 cell adhesion molecule (CD171) represents a strongly unfavorable prognostic biomarker for ovarian and endometrial carcinomas. Here we carried out an immunohistochemical survey of L1 expression in normal adults and in a broad range of benign and malignant tumors using monoclonal antibody L1-11A and the novel monoclonal antibody L1-14.10. In normal tissues, L1 was expressed in the collecting tubules of adult tissues and pediatric kidney and in peripheral nerve bundles. In tumors of the female genital tract, L 1 was detected in adenocarcinomas of the cervix and fallopian tubes, in addition to ovarian and endometrial carcinomas. Nongynecological tumors expressing L1 comprised malignant melanoma, colon adenocarcinoma positive to chromogranin, clear-cell adenocarcinoma of the urinary bladder, pheochromocytoma, small cell lung carcinoma, and tumors of the nervous system. L1 was absent in breast carcinoma, gastrointestinal tract carcinomas, gastrointestinal carcinoids, renal clear-cell carcinomas, prostate adenocarcinomas, and mesotheliomas. Surprisingly, L1 expression in established breast and renal carcinoma cell lines was not a predictor for its presence in these human tumors in vivo. Our results suggest that L1 expression in tumors is not ubiquitous but restricted to certain subtypes and may be a helpful molecular marker for differential diagnosis and target for antibody-based therapy. (c) 2006 Elsevier Inc. All rights reserved.