Identification and first insights into the structure and biosynthesis of chitin from the freshwater sponge Spongilla lacustris
Identification and first insights into the structure and biosynthesis of chitin from the freshwater sponge Spongilla lacustris
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DOI：
10.1016/j.jsb.2013.06.015
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发表时间：
2013-09-01
影响因子：
3
通讯作者：
Woerheide, Gert
中科院分区：
文献类型：
--
作者：
Ehrlich, Hermann;Kaluzhnaya, Oksana V.;Woerheide, Gert
这项工作表明，甲壳素是一个重要的结构成分内的骨骼纤维的淡水海绵海绵。使用各种分析技术（C-13固态NMR，FT-IR，拉曼，NEXAFS，ESI-MS，摩根-埃尔森测定和Calcofluor白色染色），我们表明，这种海绵几丁质是更接近α-几丁质，已知存在于其他动物，比β-几丁质。遗传分析证实了几丁质酶的存在，这是第一次在海绵中描述。甲壳素存在于海洋（demosponges和hexactinellids）和淡水海绵表明，这种重要的结构生物聚合物已经存在于他们的共同祖先。(C)2013 Elsevier Inc. All rights reserved.
This work demonstrates that chitin is an important structural component within the skeletal fibers of the freshwater sponge Spongilla lacustris. Using a variety of analytical techniques (C-13 solid state NMR, FT-IR, Raman, NEXAFS, ESI-MS, Morgan-Elson assay and Calcofluor White Staining); we show that this sponge chitin is much closer to alpha-chitin, known to be present in other animals, than to beta-chitin. Genetic analysis confirmed the presence of chitin synthases, which are described for the first time in a sponge. The presence of chitin in both marine (demosponges and hexactinellids) and freshwater sponges indicates that this important structural biopolymer was already present in their common ancestor. (C) 2013 Elsevier Inc. All rights reserved.