Matsutake -: morphological evidence of ectomycorrhiza formation between Tricholoma matsutake and host roots in a pure Pinus densiflora forest stand
Matsutake -: morphological evidence of ectomycorrhiza formation between Tricholoma matsutake and host roots in a pure Pinus densiflora forest stand
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DOI：
10.1046/j.1469-8137.2000.00707.x
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发表时间：
2000-08-01
期刊：
影响因子：
9.4
通讯作者：
Suzuki, K
中科院分区：
文献类型：
--
作者：
Gill, WM;Guerin-Laguette, A;Suzuki, K
多年来，关于松茸的营养地位一直存在着相互矛盾的争论。本文研究了采自日本赤松纯林内的松口蘑（Tricholoma matsutake Shire）的密花芬兰根的形态、结构和超微结构。松茸是夏尔和殖民根尖内的高度优势真菌，这表明报道的根形态改变可以归因于T.松茸感染松茸殖民根的外部形态与以前的描述是一致的。根外菌丝、外套膜和皮层内哈氏网菌丝的存在清楚地表明T.在自然发生的夏尔，松菌与密花松菌形成外生菌根。阐明，第一次，在宿主-真菌界面的哈氏网超微结构提供了进一步的和令人信服的证据，传统的外生菌根协会。进行性变黑，观察到从基地到尖端的优势菌根类型，由于多酚和随后的坏死沉积增加，似乎是感染的结果。然而，高度成核的维管组织的存在表明在这些根承载坏死皮层的维管圆柱体的活力。在定植根中黑色坏死皮层组织的优势可能反映了T.松茸。
There has been conflicting debate over many years regarding the trophic status of Matsutake. Here we examined the morphology, structure and ultrastructure of Finns densiflora roots collected from a Tricholoma matsutake Shire within a pure Japanese red pine stand. Molecular investigations (PCR-RFLP analyses) indicated that T. matsutake was the highly dominant fungus within both the Shire and the colonized root tips, suggesting that reported root morphology modifications can be attributed to T. matsutake infection. The external morphology of Matsutake-colonized roots is consistent with previous descriptions. The presence of extraradical mycelium, mantle, and intracortical Hartig net hyphae indicates clearly that T. matsutake forms an ectomycorrhizal association with P. densiflora in naturally occurring Shires. The elucidation, for the first time, of the Hartig net ultrastructure at the host-fungus interface provides further and convincing evidence of a conventional ectomycorrhizal association. The progressive blackening, observed from base to tip in dominant mycorrhizal types, due to increased deposition of polyphenol and subsequent necrosis, appears to be a result of infection. However, the presence of highly nucleated vascular tissue indicates the viability of the vascular cylinder in these roots bearing necrotic cortices. Such a preponderance of black necrotic cortical tissues among colonized roots may reflect some atypical behaviour of T. matsutake.