Phylogenomic Relationships of Rice Oxalate Oxidases to the Cupin Superfamily and Their Association with Disease Resistance QTL
Phylogenomic Relationships of Rice Oxalate Oxidases to the Cupin Superfamily and Their Association with Disease Resistance QTL
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DOI：
10.1007/s12284-009-9024-0
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发表时间：
2009-03-01
期刊：
影响因子：
5.5
通讯作者：
Cruz, Casiana M. Vera
中科院分区：
文献类型：
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作者：
Carrillo, Maria Gay C.;Goodwin, Paul H.;Cruz, Casiana M. Vera
水稻草酸氧化酶基因（OXO）可能在稻瘟病菌抗性中起一定作用。基因组分析表明，4个串联重复的OXO基因，OsOXO 1-OsOXO 4，定位于第3染色体上的一个抗稻瘟病QTL。这些基因具有>90%的核苷酸和氨基酸同一性，但与日本晴基因组中cupin超家族的70个其他成员相比，它们具有独特的基因结构、保守的基序和同源性，这些成员被分为几个类别。在抗、感Vandana/Moroberekan高代回交系中，OsOXO 4在水稻-M.互作，其表达增加早于感病株系。OsOXO 4在抗性株系中的早期表达与含有细菌应答性转录顺式元件的额外拷贝的26-bp启动子插入相关。我们的研究结果表明OsOXO 1 -4是一类独立的水稻cupin基因，支持OsOXO 4启动子变体在抗稻瘟病中的作用。米。
Rice oxalate oxidase genes (OXO) may play a role in resistance to Magnaporthe oryzae. Genome analyses showed four tandemly duplicated OXO genes, OsOXO1-OsOXO4, which mapped to a blast resistance QTL in chromosome 3. These genes have >90% nucleotide and amino acid identity, but they have unique gene structures, conserved motifs, and phylogeny compared to the 70 other members of the cupin superfamily in the Nipponbare genome, which were divided into several classes. In resistant and susceptible Vandana/Moroberekan advanced backcross lines, only OsOXO4 was expressed during rice-M. oryzae interactions, and its expression increased earlier in resistant than susceptible lines. The earlier expression of OsOXO4 in resistant lines correlated with a 26-bp promoter insertion containing an additional copy of the bacterial responsive nodulation cis-element. Our results showed that OsOXO1-4 are in a separate class of rice cupin genes and supports a role for the promoter variant of OsOXO4 in resistance to M. oryzae.