Garlicin Post-Conditioning Suppresses Adhesion Molecules in a Porcine Model of Myocardial Ischemia-Reperfusion Injury
Garlicin Post-Conditioning Suppresses Adhesion Molecules in a Porcine Model of Myocardial Ischemia-Reperfusion Injury
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DOI：
10.1007/s11655-017-2757-5
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发表时间：
2019-01-01
影响因子：
2.9
通讯作者：
Li Xian-lun
中科院分区：
文献类型：
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作者：
Yang Peng;Li Jia-hui;Li Xian-lun
目的探讨大蒜素后处理是否能通过影响粘附分子整合素1/CD 29和血小板内皮细胞粘附分子1（PECAM-1/CD 31）减轻急性心肌梗死（AMI）猪模型心肌缺血再灌注损伤。模型组和大蒜素组建立猪AMI模型。模型组和大蒜素组动物用扩张球囊闭塞冠状动脉左前降支远端2 h，再灌注3 h。大蒜素组在再灌注前1 h内注射大蒜素1.88 mg/kg。采用实时荧光定量PCR、免疫组化和Western blot方法检测再灌注3 h后心肌组织中CD 29和CD 31的mRNA和蛋白表达。与模型组相比，大蒜素可抑制再灌注区和非再灌注区CD 29和CD 31的mRNA和蛋白表达（P
ObjectivesTo evaluate whether garlicin post-conditioning can attenuate myocardial ischemiareperfusion injury in a catheter-based porcine model of acute myocardial infarction (AMI) by affecting adhesion molecules integrin 1/CD29 and platelet endothelial cell adhesion molecule-1 (PECAM-1/CD31).MethodsTwenty-two swine were devided into 3 groups: 6 in a sham-operation group, and 8 each in the model and garlicin groups. AMI porcine model was established in the model and garlicin groups. The distal parts of the left anterior descending coronary artery in the animals of the model and garlicin groups were occluded by dilated balloon for 2 h, followed by reperfusion for 3 h. Garlicin (1.88 mg/kg) was injected over a period of 1 h, beginning just before reperfusion, in the garlicin group. Real-time polymerase chain reaction, immunohistochemistry and Western blot were carried out to detect mRNA and protein expressions of CD29 and CD31 3 h after reperfusion.ResultsHematoxylin-eosin staining showed a better myocardial structure in the garlicin group after reperfusion. Compared to the model group, garlicin inhibited both the mRNA and protein expression of CD29 and CD31 in reperfusion area and no-reflflow area (P