Molecular typing of Treponema pallidum isolates from Buenos Aires, Argentina: Frequent Nichols-like isolates and low levels of macrolide resistance.
Molecular typing of Treponema pallidum isolates from Buenos Aires, Argentina: Frequent Nichols-like isolates and low levels of macrolide resistance.
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DOI：
10.1371/journal.pone.0172905
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发表时间：
2017
期刊：
影响因子：
3.7
通讯作者：
Šmajs D
中科院分区：
文献类型：
--
作者：
Gallo Vaulet L;Grillová L;Mikalová L;Casco R;Rodríguez Fermepin M;Pando MA;Šmajs D
2006年和2013年在阿根廷布宜诺斯艾利斯共收集了54份临床样本，包括诊断为梅毒的患者的生殖器病变拭子、全血和脑脊液。43份样本（79.6%）检测到密螺旋体DNA，并采用基于测序的分子分型（SBMT）和增强cdc分型（ECDCT）进行进一步分析。通过SBMT， 10种不同的梅毒螺旋体亚种。其中，6个基因型与TPA SS14菌株相关，4个基因型与TPA Nichols菌株相关。在42例患者中扩增到23S rRNA基因，其中6例（14.3%）发现A2058G（4例，9.5%）或A2059G（2例，4.8%）突变。除台湾、马达加斯加和秘鲁外，阿根廷是尼科尔斯样分离株流行率（26.8%）大于10%的另一个国家。
A total of 54 clinical samples, including genital lesion swabs, whole blood and cerebrospinal fluid from patients diagnosed with syphilis were collected in 2006 and in 2013 in Buenos Aires, Argentina. Treponemal DNA was detected in 43 of the analyzed samples (79.6%) and further analyzed using Sequencing-based molecular typing (SBMT) and Enhanced CDC-typing (ECDCT). By SBMT, 10 different Treponema pallidum subsp. pallidum (TPA) genotypes were found, of which six were related to the TPA SS14 strain, and four to the TPA Nichols strain. The 23S rRNA gene was amplified in samples isolated from 42 patients, and in six of them (14.3%), either the A2058G (four patients, 9.5%) or the A2059G (two patients, 4.8%) mutations were found. In addition to Taiwan, Madagascar and Peru, Argentina is another country where the prevalence of Nichols-like isolates (26.8%) is greater than 10%.