Localization-based super-resolution microscopy with an sCMOS camera
Localization-based super-resolution microscopy with an sCMOS camera
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使用 sCMOS 相机的基于定位的超分辨率显微镜
DOI：
10.1364/oe.19.019156
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发表时间：
2011-09-26
期刊：
影响因子：
3.8
通讯作者：
Zeng, Shaoqun
中科院分区：
文献类型：
--
作者：
Huang, Zhen-Li;Zhu, Hongyu;Zeng, Shaoqun
在基于定位的超分辨率显微镜（或称为定位显微镜）的社区中，通常认为单个分子的发射是如此微弱，以至于需要使用EMCCD（电子倍增电荷耦合器件）相机作为检测器，以消除读取噪声。在这里，我们评估了一种新的弱光探测器，科学互补金属氧化物半导体（sCMOS）相机在本地化显微镜的可能性。我们的实验表明，sCMOS是能够成像肌动蛋白束的半高宽直径为37 nm，证明了sCMOS在本地化显微镜的能力。我们进一步表征了sCMOS的噪声性能，并发现，使用明亮的荧光探针，如d2EosFP，定位显微成像现在工作在散粒噪声有限的区域。(C)2011年美国光学学会
In the community of localization-based super-resolution microscopy (or called localization microscopy), it is generally believed that the emission of single molecules is so weak that an EMCCD (electron multiplying charge coupled device) camera is necessary to be used as the detector by eliminating read noise. Here we evaluate the possibility of a new kind of low light detector, scientific complementary metal-oxide-semiconductor (sCMOS) camera in localization microscopy. We demonstrate experimentally that sCMOS is capable of imaging actin bundles with FWHM diameter of 37 nm, evidencing the capability of sCMOS in localization microscopy. We further characterize the noise performance of sCMOS and find out that, with the use of a bright fluorescence probe such as d2EosFP, localization microscopy imaging is now working in the shot noise limited region. (C) 2011 Optical Society of America