Direct assessment of histone function using histone replacement.
Direct assessment of histone function using histone replacement.
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DOI：
10.1016/j.tibs.2022.06.010
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发表时间：
2022-07
影响因子：
13.8
通讯作者：
Emma Tung Corcoran;Yannick Jacob
中科院分区：
文献类型：
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作者：
Emma Tung Corcoran;Yannick Jacob
组蛋白在真核细胞中用于多种目的，调节不同的基因组过程，包括转录、复制、DNA修复和染色质组织。因此，评估组蛋白功能的实验系统是阐明染色质上发生的活动调节的基本资源。研究组蛋白功能的一组重要工具是组蛋白替代系统，其中内源性组蛋白表达可以部分或完全被突变组蛋白替代。组蛋白替代系统允许系统地筛选组蛋白调节功能和直接评估组蛋白残基的功能。在这篇综述中，我们描述了现有的组蛋白替代系统的模式生物，这些系统的优点和局限性，以及未来的研究与组蛋白替代策略的机会。
Histones serve many purposes in eukaryotic cells in the regulation of diverse genomic processes, including transcription, replication, DNA repair, and chromatin organization. As such, experimental systems to assess histone function are fundamental resources toward elucidating the regulation of activities occurring on chromatin. One set of important tools for investigating histone function are histone replacement systems, in which endogenous histone expression can be partially or completely replaced with a mutant histone. Histone replacement systems allow systematic screens of histone regulatory functions and the direct assessment of functions for histone residues. In this review, we describe existing histone replacement systems in model organisms, the benefits and limitations of these systems, and opportunities for future research with histone replacement strategies.