Characterization of the cellular binding of exogenous gangliosides.
Characterization of the cellular binding of exogenous gangliosides.
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外源神经节苷脂的细胞结合的表征。
DOI：
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发表时间：
1977
期刊：
影响因子：
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通讯作者：
Herbert Wiegandt
中科院分区：
文献类型：
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作者：
Richard Callies;Gunter Schwarzmann;Klaus Radsak;Rudolf Siegert;Herbert Wiegandt
实验进行表征不同外源性唾液糖脂的细胞积累。
Experiments were performed to characterize the accumulation of different exogenous sialoglycolipids by cells.
A biphasic kinetics of accumulation appeared to be characteristic for intact primary and permanent monolayer cells and chicken erythrocytes whereas that of chicken erythrocyte ghosts was monophasic. Saturation levels could be reached with Galβ13 GalNAcβ14 Gal[32αNeuAc]-β14 Glcβ1–1′Cer and Galβ13 GalNAcβ14 Gal[32αNeuAc82αNeuAc]β14 Glcβ1 – 1′Cer with concentrations of 100 and 200 nmol per ml culture medium, respectively. Under these conditions the amount of 1.3–1.4 nmol of sialoglycolipids per 100 μg of cellular protein were found to be cell-bound after approximately two hours. In case of the ganglioside NeuAcα23Galβ14 Glcβ1 – 1′Cer no saturation could be reached.
The intermediate plateau of accumulation was abolished by the agents colchicine and sodium fluoride which on the other hand had no influence on the level of saturation. In contrast the presence of cytochalasin B, 2-deoxy-D-glucose plus sodium azide, and glutardialdehyde as well as low temperature decreased the amount of incorporated gangliosides drastically. The effect of cytochalasin B was limited to monolayer cultures.
Mild trypsinisation liberated some 70–85% of cell-associated gangliosides even after drug-inhibited accumulation of gangliosides. Also some 87–95% of the cell-bound ganglioside NeuAcα23 Galβ1–4 Glcβ1–1′Cer remained accessible to the action of exogenous sialidase, thus excluding gross internalisation.