Effect of Psidium cattleianum leaf extract on Streptococcus mutans viability, protein expression and acid production
Effect of Psidium cattleianum leaf extract on Streptococcus mutans viability, protein expression and acid production
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DOI：
10.1159/000121439
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发表时间：
2008-01-01
期刊：
影响因子：
4.2
通讯作者：
ten Cate, J. M.
中科院分区：
文献类型：
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作者：
Brighenti, F. L.;Luppens, S. B. I.;ten Cate, J. M.
植物天然产生可用作抗菌剂的次级代谢物。本研究的目的是评估番石榴叶提取物对变形链球菌的作用。通过在600 ml去离子水中煎煮100 g叶获得提取物（100%）。为了评估杀人，S。用水（阴性对照）或各种提取物稀释液[ 100、50、25%（v/v）水溶液]处理变形杆菌生物膜5或60 min。为了评估对蛋白质表达的影响，将生物膜暴露于水或1.6%（v/v）提取物120 min，提取蛋白质并进行二维差异凝胶电泳。通过质谱鉴定差异表达的蛋白质。通过pH测量确定1.6%（v/v）提取物对酸产生的影响，并与水对照进行比较。用100%浸提液处理5分钟或用50%浸提液处理60分钟后，存活率相似（约0.03%存活率）。处理60分钟后，暴露于25%或50%浸提液的生物膜之间的存活率没有差异（约0.02%存活率）。用1.6%提取物处理显著改变了蛋白质表达。24个斑点的丰度与水相比降低（p < 0.05）。该提取物显著抑制产酸（p < 0.05）。因此，卡特兰叶提取物对S.当以高浓度施用时，在生物膜中生长的变异体。在低浓度下，它抑制S。变形酸的产生，并减少参与一般代谢、糖酵解和乳酸产生的蛋白质的表达。版权所有（C）2008 S. Karger AG，巴塞尔。
Plants naturally produce secondary metabolites that can be used as antimicrobials. The aim of this study was to assess the effects of Psidium cattleianum leaf extract on Streptococcus mutans. The extract (100%) was obtained by decoction of 100 g of leaves in 600 ml of deionized water. To assess killing, S. mutans biofilms were treated with water (negative control) or various extract dilutions [ 100, 50, 25% (v/v) in water] for 5 or 60 min. To evaluate the effect on protein expression, biofilms were exposed to water or 1.6% (v/v) extract for 120 min, proteins were extracted and submitted to 2-dimensional difference gel electrophoresis. Differentially expressed proteins were identified by mass spectrometry. The effect of 1.6% (v/v) extract on acid production was determined by pH measurements and compared to a water control. Viability was similar after 5 min of treatment with the 100% extract or 60 min with the 50% extract (about 0.03% survival). There were no differences in viability between the biofilms exposed to the 25 or 50% extract after 60 min of treatment (about 0.02% survival). Treatment with the 1.6% extract significantly changed protein expression. The abundance of 24 spots was decreased compared to water (p < 0.05). The extract significantly inhibited acid production (p < 0.05). It is concluded that P. cattleianum leaf extract kills S. mutans grown in biofilms when applied at high concentrations. At low concentrations it inhibits S. mutans acid production and reduces the expression of proteins involved in general metabolism, glycolysis and lactic acid production. Copyright (C) 2008 S. Karger AG, Basel.