Expression of receptor activator of NF-kappa B ligand and osteoprotegerin in culture of human periodontal ligament cells
Expression of receptor activator of NF-kappa B ligand and osteoprotegerin in culture of human periodontal ligament cells
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DOI：
10.1034/j.1600-0765.2002.01603.x
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发表时间：
2002-12-01
影响因子：
3.5
通讯作者：
Shirakawa, T
中科院分区：
文献类型：
--
作者：
Hasegawa, T;Yoshimura, Y;Shirakawa, T
NF-κ B 配体受体激活剂 (RANKL) 及其诱饵受体骨保护素 (OPG) 是与破骨细胞生成有关的重要蛋白质。在本研究中，我们研究了 RANKL 和 OPG 在培养的人牙周膜 (PDL) 细胞中的表达及其在破骨细胞生成中的作用。 Northern blotting显示，使用10(-8) m one-α, 25-二羟基维生素D-3 [1,25-(OH)(2)D-3]和10(-7) m 地塞米松(Dex)后，OPG mRNA显着下调。相比之下，同样的处理使 RANKL mRNA 上调。蛋白质印迹证明使用 1,25-(OH)(2)D-3 和 Dex 可以降低 OPG。当 PDL 细胞与小鼠骨髓细胞在抗 OPG 抗体以及 1,25-(OH)(2)D-3 和 Dex 存在下共培养时，显着诱导酒石酸抗性酸性磷酸酶阳性多核细胞。这些结果表明PDL细胞合成RANKL和OPG，OPG失活可能在破骨细胞的分化中发挥关键作用。
The receptor activator of NF-kappa B ligand (RANKL) and its decoy receptor, osteoprotegerin (OPG), are the important proteins implicated in osteoclastogenesis. In this study, we investigated the expressions of RANKL and OPG in cultured human periodontal ligament (PDL) cells and their roles in osteoclastogenesis. Northern blotting revealed that the OPG mRNA was downregulated remarkably by application of 10(-8) m one-alpha, 25-dihydroxyvitamin D-3 [1,25-(OH)(2)D-3] and 10(-7) m dexamethasone (Dex). In contrast, RANKL mRNA was up-regulated by the same treatment. Western blotting demonstrated decrease of OPG by the application of 1,25-(OH)(2)D-3 and Dex. Tartrate-resistant acid phosphatase-positive multinuclear cells were markedly induced when the PDL cells were cocultured with mouse bone marrow cells in the presence of an anti-OPG antibody together with 1,25-(OH)(2)D-3 and Dex. These results indicate that PDL cells synthesize both RANKL and OPG and that inactivation of OPG may play a key role in the differentiation of osteoclasts.