The localization of acetylcholinesterase in the cochlea.
The localization of acetylcholinesterase in the cochlea.
复制标题
乙酰胆碱酯酶在耳蜗中的定位。
DOI：
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发表时间：
1959
期刊：
影响因子：
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通讯作者：
R. Doran
中科院分区：
文献类型：
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作者：
H. Schuknecht;J. A. Churchill;R. Doran
简介 最近我们报告了实验结果 3,17，证明乙酰胆碱酯酶 (AChE) 存在于耳蜗内的神经纤维中。采用Koelle 的方法进行AChE 的组织化学检测。该技术被应用于去除外骨壁后的猫的孤立耳蜗。然后将部分骨螺旋板和柯蒂氏器放在载玻片上进行研究。简而言之，组织化学方法（在其他地方 8-10 中有详细描述）包括在铜离子存在下在乙酰硫胆碱溶液中孵育组织。酶水解释放的硫代胆碱立即与铜离子形成沉淀。当用硫化铵处理样品时，产物硫代胆碱铜随后转化为硫化铜。因此，在 AChE 所在的位置可以看到硫化铜，一种稳定且可见的沉淀物。我们确信 AChE 是存在于耳蜗中的物质
Introduction Recently we reported the results of experiments 3,17 demonstrating that acetylcholinesterase (AChE) was present in the nerve fibers within the cochlea. Koelle's method for the histochemical detection of AChE was employed. The technique was applied to the isolated cochleas of cats after removal of the outer bony wall. Portions of the osseous spiral lamina and organ of Corti were then teased onto glass slides for study. In brief, the histochemical method, described fully elsewhere, 8-10 consisted of incubating tissues in a solution of acetylthiocholine in the presence of cupric ion. The thiocholine liberated by enzymatic hydrolysis immediately formed a precipitate with cupric ions. The product, copper thiocholine, was converted later to copper sulfide when the specimen was treated with ammonium sulfide. Thus, copper sulfide, a stable and visible precipitate, was seen where AChE had been located. We satisfied ourselves that AChE was the material present in the cochlea by