Cell cycle regulation of c-Jun N-terminal kinase activity at the centrosomes.
Cell cycle regulation of c-Jun N-terminal kinase activity at the centrosomes.
复制标题
DOI：
10.1006/bbrc.2001.5948
复制
发表时间：
2001-11
影响因子：
3.1
通讯作者：
Rebecca A. MacCorkle-Chosnek;Aaron VanHooser;David W. Goodrich;B. Brinkley;T. Tan
中科院分区：
文献类型：
--
作者：
Rebecca A. MacCorkle-Chosnek;Aaron VanHooser;David W. Goodrich;B. Brinkley;T. Tan
C-Jun氨基末端激酶(JNK)是丝氨酸/苏氨酸激酶(MAPK)家族中丝裂原活化蛋白激酶(MAPK)家族的一个亚类，在细胞生长和凋亡中发挥重要作用。虽然机制尚不清楚，但JNK也参与了启动细胞周期检查点和细胞周期进程的信号通路。通过跟踪细胞周期中活性和非活性JNK的定位，我们发现大多数细胞JNK是可溶的，存在于细胞质和细胞核中。有趣的是，JNK的不溶性部分也定位于核斑点和细胞质斑点，以及中心体。虽然JNK在整个细胞周期中都与中心体有关，但它只在S期至细胞后期的中心体处活跃。中心体JNK的这种新的定位可能是JNK激活刺激和中心体或细胞周期事件之间的一种联系。
The c-Jun N-terminal kinase (JNK), a subgroup of the mitogen-activated protein kinase (MAPK) family of serine/threonine kinases, has established functions in cell growth and apoptosis. While the mechanisms are unclear, JNK has also been also implicated in signaling pathways that initiate cell cycle checkpoints and cell cycle progression. By following the localization of active and inactive JNK during the cell cycle, we have found that the majority of cellular JNK is soluble and present in the cytoplasm and the nucleus. Interestingly, insoluble fractions of JNK are also localized in nuclear and cytoplasmic speckles, and to the centrosomes. While JNK is associated with the centrosome throughout the cell cycle, it is only active at the centrosome from S phase through anaphase. This novel localization of centrosomal JNK is a possible link between JNK-activating stimuli and centrosome or cell cycle events.