High-efficiency ligation and recombination of DNA fragments by vertebrate cells.
High-efficiency ligation and recombination of DNA fragments by vertebrate cells.
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DOI：
10.1126/science.6301012
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发表时间：
1983-05
期刊：
影响因子：
56.9
通讯作者：
C. Miller;H. Temin
中科院分区：
文献类型：
--
作者：
C. Miller;H. Temin
DNA介导的基因转移（转染）用于将特定基因引入脊椎动物细胞。通过测定来自禽类逆转录病毒的DNA和该DNA的亚基因组片段的混合物的感染性，定量分析转染后不久的事件。用两个DNA分子转染的限制性步骤是由单个细胞摄取处于生物活性状态的两个DNA。转染的细胞以接近100%的效率介导物理上未连接的DNA的连接和重组。
DNA-mediated gene transfer (transfection) is used to introduce specific genes into vertebrate cells. Events soon after transfection were quantitatively analyzed by determining the infectivity of the DNA from an avian retrovirus and of mixtures of subgenomic fragments of this DNA. The limiting step of transfection with two DNA molecules is the uptake by a single cell of both DNA's in a biologically active state. Transfected cells mediate ligation and recombination of physically unlinked DNA's at nearly 100 percent efficiency.