BACTERIAL COMMUNITIES IN AN ULTRAPURE WATER CONTAINING STORAGE TANK OF A POWER PLANT
BACTERIAL COMMUNITIES IN AN ULTRAPURE WATER CONTAINING STORAGE TANK OF A POWER PLANT
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DOI：
10.1556/amicr.58.2011.4.12
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发表时间：
2011-12-01
影响因子：
1.5
通讯作者：
Toth, Erika M.
中科院分区：
文献类型：
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作者：
Bohus, Veronika;Keki, Zsuzsa;Toth, Erika M.
含有低水平有机和无机化合物的超纯水(UPWs)提供了极端的环境。相反，微生物存在于这样的水域中，并在表面形成生物膜，因此在许多工业应用中可能会导致腐蚀过程。在我们的研究中，用培养和培养无关的方法对匈牙利一家发电厂锅炉生产的精制无盐水(UPW)在储存前后(对储罐的进[TKE]和[TKU]水采样)进行了检测。我们的结果显示，储存后CFU和直接细胞计数增加。培养结果表明，在两个样品中都以好氧、化学有机营养的α-变形杆菌为主。对于TKU样品，可以检测到更复杂的细菌群落结构。应用分子生物学方法(T-RFLP)分析表明，在进水(TKE)中，α-变形杆菌(Sphingomonas sp.，Novphingobium hassiacum)占主导地位，而在出水(TKU)中，细菌群落向β-变形杆菌(Rhodoferax sp.、多核细菌sp.、Sterolibacter sp.)、CFB(Bacteroidetes，前身为Cytophaga-黄杆菌-杆菌群)和浮游杆菌类群转变。这种向发酵群落方向的转变表明，储存可以帮助具有更大腐蚀倾向的群落的发展。
Ultrapure waters (UPWs) containing low levels of organic and inorganic compounds provide extreme environment. On contrary to that microbes occur in such waters and form biofilms on surfaces, thus may induce corrosion processes in many industrial applications. In our study, refined saltless water (UPW) produced for the boiler of a Hungarian power plant was examined before and after storage (sampling the inlet [TKE] and outlet [TKU] waters of a storage tank) with cultivation and culture independent methods. Our results showed increased CFU and direct cell counts after the storage. Cultivation results showed the dominance of aerobic, chemoorganotrophic alpha-Proteobacteria in both samples. In case of TKU sample, a more complex bacterial community structure could be detected. The applied molecular method (T-RFLP) indicated the presence of a complex microbial community structure with changes in the taxon composition: while in the inlet water sample (TKE) alpha-Proteobacteria (Sphingomonas sp., Novosphingobium hassiacum) dominated, in the outlet water sample (TKU) the bacterial community shifted towards the dominance of beta-Proteobacteria (Rhodoferax sp., Polynucleobacter sp., Sterolibacter sp.), CFB (Bacteroidetes, formerly Cytophaga-Flavobacterium-Bacteroides group) and Firmicutes. This shift to the direction of fermentative communities suggests that storage could help the development of communities with an increased tendency toward corrosion.