Follicular fluid and human granulosa cell cultures: influence on sperm kinetic parameters, hyperactivation, and acrosome reaction
Follicular fluid and human granulosa cell cultures: influence on sperm kinetic parameters, hyperactivation, and acrosome reaction
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DOI：
10.1016/s0015-0282(97)00421-4
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发表时间：
1998-01-01
影响因子：
6.7
通讯作者：
Flamigni, C
中科院分区：
文献类型：
--
作者：
Fabbri, R;Porcu, E;Flamigni, C
目的：评价人颗粒细胞（GC）培养物和卵泡液（FF）对精子动力学参数、超活化和顶体反应的影响，并与人输卵管液（HTF）和Ham's F-10培养基的影响进行比较。设计：在HTF、Ham's F-10培养基、FF和GC培养物中孵育6小时后，评价精子动力学参数、超活化和顶体反应。设置：妇产科研究所生殖内分泌科不孕不育与体外受精中心。患者：16份正常精液样本。干预措施：精子动力学参数和超活化用自动显微摄像系统进行分析，顶体反应用三重染色技术进行，用市售试剂盒测定孕酮和17 OH-孕酮水平。主要结果测量：精子动力学参数，超活化，顶体反应。结果：与HTF或Ham's F-10培养基中孵育相比，FF或GC培养物中孵育6小时后，能动精子的百分比、平均曲线速度和头部侧向运动的最大幅度的平均值显著增加，而平均线性度显著降低。卵泡液和GC文化显着增加超活化和顶体反应相比，使用HTF和Ham的F-10培养基获得的值。与HTF和Ham's F-10 medium. Conclusions（s）相比，在FF和GC培养物中孵育后，孕酮和17 OH-孕酮水平显著增加：卵泡液和GC培养物增加精子运动参数、超活化和顶体反应。这种效应可能与GC对微囊藻毒素的解毒作用或GC分泌肽、糖蛋白、生长因子（胰岛素样生长因子1和2）或类固醇（孕酮和17 OH-孕酮）有关。(C)1998年，美国生殖医学会。
Objective: To evaluate the influence of human granulosa cell (GC) cultures and follicular fluid (FF) on sperm kinetic parameters, hyperactivation, and the acrosome reaction compared with the influence of human tubal fluid (HTF) and Ham's F-10 medium.Design: Sperm kinetic parameters, hyperactivation, and the acrosome reaction were evaluated after 6 hours of incubation in HTF, Ham's F-10 medium, FF, and GC cultures.Setting: Infertility and In Vitro Fertilization Centre, Reproductive Endocrinology Unit, Institute of Obstetrics and Gynaecology.Patient(s): Sixteen normal semen samples.Intervention(s): Sperm kinetic parameters and hyperactivation were analyzed using an automated videomicrography system, the acrosome reaction was performed using a triple-stain technique, and progesterone and 17OH-progesterone levels were measured with the use of commercially available kits.Main Outcome Measure(s): Sperm kinetic parameters, hyperactivation, acrosome reaction.Result(s): The percentage of-motile sperm, the mean curvilinear velocity, and the mean of the maximum amplitude of lateral head movement were increased significantly after 6 hours of incubation in FF or GC cultures compared with incubation in HTF or Ham's F-10 medium, whereas the mean linearity was decreased significantly. Follicular fluid and GC cultures significantly increased hyperactivation and the acrosome reaction compared with the values obtained using HTF and Ham's F-10 medium. Progesterone and 17OH-progesterone levels were increased significantly after incubation in FF and GC cultures compared with HTF and Ham's F-10 medium.Conclusion(s): Follicular fluid and GC cultures increase sperm motility parameters, hyperactivation, and the acrosome reaction. This effect may be related to GC detoxification of the microenvironement of the microenvironement or GC secretion of peptides, glycoproteins, growth factors (insulin-like growth factors 1 and 2), or steroids (progesterone and 17OH-progesterone). (C) 1998 by American Society for Reproductive Medicine.