THE LEADER SEQUENCE OF THE SUBGENOMIC MESSENGER-RNAS OF ROUS-SARCOMA VIRUS IS APPROXIMATELY 390 NUCLEOTIDES
THE LEADER SEQUENCE OF THE SUBGENOMIC MESSENGER-RNAS OF ROUS-SARCOMA VIRUS IS APPROXIMATELY 390 NUCLEOTIDES
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DOI：
10.1128/jvi.41.2.527-534.1982
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发表时间：
1982-01-01
影响因子：
5.4
通讯作者：
BISHOP, JM
中科院分区：
文献类型：
--
作者：
HACKETT, PB;SWANSTROM, R;BISHOP, JM
劳斯肉瘤病毒的亚基因组mRNA共享从基因组RNA剪接的共同5“前导序列。本文对4种Rous肉瘤病毒RNA的5“末端序列进行了检测，并与3种细胞内指导RSV基因产物合成的mRNA进行了比较。RNA上前导序列的长度取决于它们在RNA-DNA杂交后保护克隆的劳斯肉瘤病毒DNA片段不被S1核酸酶消化的程度。发现指导env和src基因产物合成的亚基因组mRNA具有不间断的剪接前导序列。390个核苷酸;病毒体RNA和全长细胞内病毒RNA具有与克隆的病毒DNA探针在至少前735个碱基上同源的5“末端。测定了劳斯肉瘤病毒基因组5“末端的核苷酸序列，包括此处鉴定的候选剪接供体位点。
The subgenomic mRNA of Rous sarcoma virus share a common 5'' leader sequence spliced from genomic RNA. The 5'' terminal sequences of 4 Rous sarcoma virus RNA were examined virion RNA and 3 species of intracellular mRNA which direct the synthesis of the RSV gene products. The lengths of the leaders on the RNA were determined by the extent that they could protect cloned Rous sarcoma virus DNA fragments from S1 nuclease digestion after RNA-DNA hybridization. The subgenomic mRNA that direct the synthesis of the env and src gene products were found to have uninterrupted spliced leader sequences of .apprx. 390 nucleotides; virion RNA and full-length intracellular viral RNA have 5'' termini homologous to the cloned viral DNA probe over at least the first 735 bases. The nucleotide sequence of the 5'' end of the Rous sarcoma virus genome, including the candidate splice donor site identified here was determined.