OCCURRENCE OF BIOCHEMICAL METAMORPHIC EVENTS WITHOUT ANATOMICAL METAMORPHOSIS IN AXOLOTL
OCCURRENCE OF BIOCHEMICAL METAMORPHIC EVENTS WITHOUT ANATOMICAL METAMORPHOSIS IN AXOLOTL
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DOI：
10.1016/0012-1606(74)90268-1
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发表时间：
1974-01-01
影响因子：
2.7
通讯作者：
DUCIBELLA, T
中科院分区：
文献类型：
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作者：
DUCIBELLA, T
墨西哥钝口螈虽然在解剖学上是一种幼体两栖动物，但它确实产生少量的内源性甲状腺素。根据不同组织对甲状腺素的反应阈值不同的理论，美西螈可能经历了某些生化变态事件，而没有解剖变态。凝胶电泳表明，血红蛋白和血清蛋白组成的血液中发生了变化，分别在约125和210天的正常发育。这两个事件都是由甲状腺素过早诱导的;变态动物具有新生期成人血红蛋白和血清蛋白电泳图谱，在十二烷基硫酸钠-聚丙烯酰胺凝胶上，变性的新生期成人血红蛋白分成分子量为15，000和19，000以及24，500的组分（相对于牛血红蛋白单体的分子量为15，500）。幼鱼血红蛋白的分子量分别为13，800和19，500，表明：（1）在正常发育的100-150天，新的血红蛋白多肽亚基的产生受到刺激，而幼鱼亚基的合成受到抑制;（2）美西螈在至少两次正常的变质事件中不是生物化学上的新生动物;（3）负责某些生化变态事件的组织可能比负责解剖变态的组织对甲状腺素的反应阈值低。
Although the axolotlAmbystoma mexicanumis anatomically a larval amphibian, it does produce a small amount of endogenous thyroxine. According to the theory that different tissues have different thresholds of response to thyroxine, the axolotl may undergo certain biochemical metamorphic events without anatomical metamorphosis.Gel electrophoresis demonstrated that the hemoglobin and serum protein composition of the blood changed at approximately 125 and 210 days of normal development, respectively. Both events were prematurely inducible by thyroxine; metamorphosed animals had neotenous adult hemoglobin and serum protein electrophoretic patterns.On sodium dodecyl sulfate-polyacrylamide gels, denatured neotenous adult hemoglobin fractionated into components of 15,000 and 19,000, and 24,500 molecular weight (relative to that of bovine hemoglobin monomer, 15,500). Larval hemoglobin possessed components of 13,800 and 19,500 molecular weight.It was concluded that (1) at 100–150 days of normal development, the production of new hemoglobin polypeptide subunits was stimulated whereas the synthesis of larval subunits was inhibited, (2) the axolotl is not biochemically neotenous for at least two normally metamorphic events, and (3) the tissues responsible for certain biochemical metamorphic events probably have a lower threshold of response to thyroxine than the tissues responsible for anatomical metamorphosis.