The 9-O-acetyl GD3 gangliosides are expressed by migrating chains of subventricular zone neurons in vitro
The 9-O-acetyl GD3 gangliosides are expressed by migrating chains of subventricular zone neurons in vitro
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DOI：
10.1590/s0100-879x2001000500016
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发表时间：
2001-05-01
影响因子：
2.3
通讯作者：
Hedin-Pereira, C
中科院分区：
文献类型：
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作者：
Miyakoshi, LM;Mendez-Otero, R;Hedin-Pereira, C
在发育过程中和成年啮齿类动物中，大脑皮层前室下区（SVZ）的神经元切向迁移成为嗅球中的中间神经元。这种迁移被定义为嗜神经元的，独立于放射状胶质基质。皮质SVZ和吻侧迁移流的嗅球被证明是丰富的9-O-乙酰GD 3神经节苷脂（9-O-acGD 3），这已被证明是涉及在啮齿动物大脑皮层和小脑中的亲胶质细胞迁移。在本研究中，我们进行了SVZ外植体培养大鼠在其出生后的第一个星期，分析这些神经节苷脂的神经元前体细胞链迁移的表达。我们通过形态学分析和神经细胞粘附分子的免疫细胞化学表征了这些成神经细胞的迁移链。通过使用与9-O-acGD 3特异性结合的Jones单克隆抗体，我们表明来自SVZ外植体的迁移链表达9-O-acGD 3，其以点状方式分布在单个细胞中。9 O-acGD 3也存在于在不存在放射状胶质细胞的情况下形成的迁移链中，这是SVZ的嗜神经元链迁移的典型。我们的数据表明，9-O-乙酰化神经节苷脂可能参与嗜神经细胞和嗜胶质细胞的迁移。
Neurons from the anterior subventricular zone (SVZ) of the cerebral cortex migrate tangentially to become interneurons in the olfactory bulb during development and in adult rodents. This migration was defined as neuronophilic, independent of a radial glial substrate. The cortical SVZ and the rostral migratory stream to the olfactory bulb were shown to be rich in 9-O-acetyl GD3 gangliosides (9-O-acGD3), which have been previously shown to be implicated in gliophilic migration in the rodent cerebral cortex and cerebellum. In the present study, we performed SVZ explant cultures using rats during their first postnatal week to analyze the expression of these gangliosides in chain migration of neuronal precursors. We characterized migrating chains of these neuroblasts through morphological analysis and immunocytochemistry for the neural cell adhesion molecule. By using the Jones monoclonal antibody which binds specifically to 9-O-acGD3 we showed that migrating chains from the SVZ explants express 9-O-acGD3 which is distributed in a punctate manner in individual cells. 9O-acGD3 is also present in migrating chains that form in the absence of radial glia, typical of the neuronophilic chain migration of the SVZ. Our data indicate that 9-O-acetylated gangliosides may participate in neuronophilic as well as gliophilic migration.