cDNA cloning of a mouse pituitary adenylate cyclase-activating polypeptide receptor
cDNA cloning of a mouse pituitary adenylate cyclase-activating polypeptide receptor
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DOI：
10.1016/0005-2736(96)00056-9
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发表时间：
1996-06-11
影响因子：
3.4
通讯作者：
Baba, A
中科院分区：
文献类型：
--
作者：
Hashimoto, H;Yamamoto, K;Baba, A
利用逆转录聚合酶链式反应（RT-PCR）从小鼠脑中获得了垂体腺苷酸环化酶激活多肽（PACAP）受体（PACAP- r）的cDNA克隆。在COS细胞中表达的重组PACAP受体结合PACAP的亲和力比血管活性肠多肽（vasactive intestinal polypeptide， VIP）高1000倍左右，PACAP通过克隆的PACAP受体刺激腺苷酸环化酶。小鼠PACAP受体由496个氨基酸组成，包含7个跨膜片段，与大鼠、牛和人PACAP- r的同源性分别为98.4%、93.0%和92.5%。
A cDNA clone for mouse pituitary adenylate cyclase-activating polypeptide (PACAP) receptor (PACAP-R) was obtained from the brain using reverse transcription-polymerase chain reaction (RT-PCR). The recombinant PACAP receptor expressed in COS cells bound PACAP with about 1000-times higher affinity than vasoactive intestinal polypeptide (VIP), and PACAP stimulated adenylate cyclase through the cloned PACAP receptor. The mouse PACAP receptor consists of 496 amino acids, contains seven transmembrane segments and has 98.4%, 93.0%, and 92.5% identity with the rat, bovine, and human PACAP-R, respectively.